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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Protein A Peroxidase Conjugated

Code

PA00-03
Size

2 mg
Price

$175.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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Dot Blot of PEROXIDASE Conjugated Protein A.
Lane 1:  Human IgG.
Lane 2:  Golden Syrian Hamster IgG.
Lane 3:  Sheep IgG.
Load:  3-fold serial dilution starting at 200 ng.
Primary Antibody:  none.
Secondary antibody:  PEROXIDASE Conjugated Protein A at 1:1000 for 60 min at RT.
Block:  1% BSA-TTBS 30 min at RT.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: HRP, peroxidase, Horseradish peroxidase protein A

Protein A Peroxidase Conjugated Properties

Description
PROTEIN A PEROXIDASE Conjugated - PA00-03
Application
ELISA : 1:20,000 - 1:200,000
Western Blot : 1:10,000 - 1:40,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Reagent Type
Protein A
Physical State
Lyophilized
Shipping condition
Ambient
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Protein A Peroxidase Conjugated Description

Background
Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Protein A Peroxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).
Purity/Specifity
Protein A Peroxidase Conjugated was prepared from chromatographically pure Protein A.  Protein A Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
FLISA, WB, IF
Reacts With
chicken
Catalog Number
203-4201

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate