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Avidin, Biotin, Streptavidin  >  Phycoerythrin Conjugated Antibodies
 

Streptavidin Phycoerythrin Conjugated

Code

S000-08
Size

1 mL
Price

$215.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Streptavidin PE, Phyco Streptavidin, SA Phycoerythrin, R-Phycoerythrin

Streptavidin Phycoerythrin Conjugated Properties

Description
STREPTAVIDIN PHYCOERYTHRIN Conjugated - S000-08
Application
IF Microscopy : 1:100 - 1:250
FlowCytometry : 1:100 - 1:250
Reagent Type
Streptavidin
Physical State
Lyophilized
Shipping condition
Ambient
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Related Links
Download CofA
Lot#  

Streptavidin Phycoerythrin Conjugated Description

Background
Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Phycoerythrin (PE) is a red-pigmented protein found in cyanobacteria and red algae. Phycoerythrin absorbs light blue-green/yellow light and emits lightly orange/yellow light.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Phycoerythrin is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of Streptavidin Phycoerythrin required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of Streptavidin Phycoerythrin may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Purity/Specifity
Streptavidin Phycoerythrin was prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin.   Free fluorochrome is removed by tandem molecular sieve chromatography.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Mouse
Catalog Number
610-1619-0500

Product Type
Secondary Antibodies;
Reacts With
Rabbit
Catalog Number
611-106-B76

Product Type
Avidin, Biotin, Streptavidin;
Applications
FLISA, IF, FC
Catalog Number
A003-02

Product Type
Avidin, Biotin, Streptavidin;
Applications
FLISA, IF
Catalog Number
S000-00

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS