14-12_peptide top banner
 
Primary Antibodies  >  Cancer Research Antibodies
 

Hif-1 alpha hydroxy P564 Antibody

Rabbit Polyclonal
NCI Collaboration
Order Offline
Western blot using Rockland's anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor).  Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation.  Control staining is shown (right panel) using conventional anti-HIF-1alpha.  The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-????The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.  Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
View All Images
Number:
100-401-A25
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Hif-1 alpha hydroxy P564 Antibody Properties

Description
Anti-Hif-1a hydroxyP564 (RABBIT) Antibody - 100-401-A25
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, monkey, mouse, rat, dog, bovine, Xenopus
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
HIF1A
Download CofA:
Lot#  
Print

Hif-1 alpha hydroxy P564 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tumor hypoxia often directly correlates with aggressive phenotype, metastasis progression and resistance to chemotherapy. HIF-1 transcription factors are dramatically induced in hypoxic areas and regulate the expression of genes necessary for tumor adaptation to conditions of low oxygen. The stabilization of HIF-1a by hypoxia is critically dependent upon the hydroxylation of certain Proline residues that exist in the oxygen-dependent degradation domain of HIF-1a. HIF factors are now considered an important therapeutic target for cancer intervention.
Synonyms
Hypoxia-inducible factor 1-alpha antibody, ARNT-interacting protein antibody, Member of PAS protein 1 antibody, Basic-helix-loop-helix-PAS protein MOP1 antibody, PAS domain-containing protein 8 antibody, Class E basic helix-loop-helix protein 78 antibody
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
Immunogen Type
Peptide
Post Translational Modification
Hydroxylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA and western blotting.  This antibody is specific for HIF-1a hydroxylated at P564.  Minimal cross-reactivity occurs with non-hydroxylated HIF-1a.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 110 kDa in size corresponding to HIF-1a hydroxyP564 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein.  Minimal reactivity occurs with the non-hydroxylated form of the protein.  A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Kageyama, Y. and Koshiji, M. (2004) Leu-574 of human HIF-1alpha is a molecular determinant of prolyl hydroxylation. FASEB J. 18(9):1028-1030. Griffiths, E.A., Pritchard, S.A., Valentine, H.R., Whitchelo, N.,  Bishop, P.W., Ebert, M.P., Price, P.M., Welch, I.M. and West, C.M. (2007)  Hypoxia-inducible factor-1alpha expression in the gastric carcinogenesis sequence and its prognostic role in gastric and  gastro-oesophageal adenocarcinomas. Br. J. Cancer 96(1):95-103. Yin, L., Kharbanda, S. and Kufe, D. (2007) Mucin 1 oncoprotein blocks hypoxia-inducible factor 1alpha activation in a survival response to hypoxia. J. Biol. Chem. 282(1):257-266.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry