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Primary Antibodies > Cancer Research Antibodies

AGAP2 Antibody

Rabbit Polyclonal

Code

200-401-946

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10,000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.

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Synonyms: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody, centaurin gamma1 antibody, Centg1 antibody

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AGAP2 Antibody Properties

Description

Anti-AGAP2 (RABBIT) Antibody - 200-401-946

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse

Clonality

Polyclonal

Application

ELISA : 1:40,000 - 1:200,000
Western Blot : 1:5,000 - 1:20,000
Other Dilution: User Optimized

Physical State

Lyophilized

Shipping condition

Ambient

Format

IgG

Label

Unconjugated

Concentration

2.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

100 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

AGAP2

AGAP2 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. AGAP2 (also known as centaurin-gamma1, ARF-GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2, Phosphatidyl-inositol-3-kinase enhancer, PIKE, GTP-binding and GTPase activating protein 2 and GGAP2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. AGAP2 protein binds

Immunogen

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This protein A purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to AGAP2 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This protein A purified antibody is directed against human AGAP2 protein. The product was purified from monospecific antiserum by protein A chromatography. A BLAST analysis was used to suggest cross reactivity with AGAP2 protein from mouse and rat based on 93% and 95% protein:protein homologies, respectively, with the immunizing sequence. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties