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Primary Antibodies > Cancer Research Antibodies

Cenexin-1 Antibody

Rabbit Polyclonal

Code

200-401-C43

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Anti-Cenexin-1 in Western Blot using Rockland Immunochemical's Protein A Purified Anti-Cenexin-1 antibody shows detection of Cenexin-1 in total cell lysates from mouse F9 embryonic carcinoma cells. Arrowheads show detection of Cenexin-1 at approximately 93kDa and Outer dense fiber protein 2 (ODF2) at approximately 70kDa. In personal communication with K. Lee, CCR-NCI, Bethesda, MD.

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Synonyms: ODF84 antibody, Outer dense fiber of sperm tail 2 antibody, Cenexin1 variant 1 antibody, KKT 4 antibody

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Cenexin-1 Antibody Properties

Description

Anti-Cenexin-1 (RABBIT) Antibody - 200-401-C43

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:500-1:10,000
Western Blot : 1:500-1:2000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.53 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

ODF2

Cenexin-1 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenexin-1 (ODF84) and cenexin-2 are splicing variants that are expressed from the same genetic locus Outer Dense Fiber protein 2 (ODF2) . Cenexin-1 and cenexin-2, regarded as centriolar proteins. Cenexin-1, a 93-kD variant of ODF2, is the major form of cennexin expressed in various somatic cells and tissues and associates with centrosomes. Cenexin-1 contains a unique C-terminal extension that appears to play a critical role in recruiting mitotic regulators such as polo-like kinase 1. In contrast, ODF2, a 70-kDa protein, is a major component of sperm tail cytoskeleton and abundantly expressed in testis. Additional evidence suggests that both of these proteins play important role in primary cilia formation. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.

Immunogen

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Anti-Cenexin-1 purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to Cenexin-1 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography. It is directed against, and shows specific reactivity for, human Cenexin-1 protein. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee, mouse, rat, bovine sources based on 100% homology with the immunizing sequence. Reactivity with Cenexin-1 protein from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Soung,N.K., Kang,Y.H., Kim,K., Kamijo,K., Yoon,H., Seong,Y.S., Kuo,Y.L., Miki,T., Kim,S.R., Kuriyama,R., Giam,C.Z., Ahn,C.H. and Lee,K.S. (2006) Requirement of hCenexin for proper mitotic functions of polo-like kinase 1 at the centrosomes. Mol. Cell. Biol. 26 (22), 8316-8335. Kierszenbaum,A.L. (2002) Keratins: unraveling the coordinated construction of scaffolds in spermatogenic cells. Mol. Reprod. Dev. 61 (1), 1-2. Petersen,C., Fuzesi,L. and Hoyer-Fender,S. (1999) Outer dense fibre proteins from human sperm tail: molecular cloning and expression analyses of two cDNA transcripts encoding proteins of approximately 70 kDa. Mol. Hum. Reprod. 5 (7), 627-635. Hoyer-Fender,S., Petersen,C., Brohmann,H., Rhee,K. and Wolgemuth,D.J. (1998) Mouse Odf2 cDNAs consist of evolutionary conserved as well as highly variable sequences and encode outer dense fiber proteins of the sperm tail. Mol. Reprod. Dev. 51 (2), 167-175. Shao,X., Murthy,S., Demetrick,D.J. and van der Hoorn,F.A. (1998) Human outer dense fiber gene, ODF2, localizes to chromosome 9q34. Cytogenet. Cell Genet. 83 (3-4), 221-223.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra