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Primary Antibodies  >  Cancer Research Antibodies
 

ASAP1 Antibody

Rabbit Polyclonal
Code

600-401-911
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Immunofluorescent microscopy using Rockland's Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src.  Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.  Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
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Synonyms: Development and differentiation enhancing factor 1 antibody, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody, ADP ribosylation factor directed GTPase activating protein 1 antibody, AMAP 1 antibody

ASAP1 Antibody Properties

Description
Anti-ASAP1 (RABBIT) Antibody - 600-401-911
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human, chicken, bovine, dog, rat, chimpanzee
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.95 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ASAP1
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Lot#  

ASAP1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes.  Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 777-787 of mouse ASAP1 protein (see below).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract.  This antibody recognizes both phosphorylated and non-phosphorylated ASAP1 at amino acid Y782.
Purity/Specifity
This affinity-purified antibody is directed against mouse ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Reactivity occurs against both the phosphorylated and non-phosphorylated forms of the protein at residue Y782.  A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Brown MT, Andrade J, Radhakrishna H, Donaldson JG, Cooper JA, Randazzo PA. (1998) ASAP1, a phospholipid-dependent Arf GTPase-activating protein that associates with and is phosphorylated by Src. Mol. Cell Bio. 18: 7038-7051. Onodera Y, Hashimoto S, Hashimoto A, Morishige M, Mazaki Y, Yamada A, Ogawa E, Adachi M, Sakurai T, Manabe T, Wada H, Matsuura N, Sabe H. Expression of AMAP1, an ArfGAP, provides novel targets to inhibit breast cancer invasive activities. EMBO J. 24 : 963-973. Liu,Y., Yerushalmi,G.M., Grigera,P.R. and Parsons,J.T. (2005) Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling. J. Biol. Chem. 280 (10), 8884-8892.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry