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Primary Antibodies > Cancer Research Antibodies

MAGP-2 Antibody

Rabbit Polyclonal

Code

600-401-937

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4° C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD

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Synonyms: MFAP 5 antibody, MFAP5 antibody, Microfibril associated glycoprotein 2 antibody, microfibrillar associated protein 5 antibod

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MAGP-2 Antibody Properties

Description

Anti-MAGP-2 (RABBIT) Antibody - 600-401-937

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, chimpanzee

Clonality

Polyclonal

Application

ELISA : 1:5,000 - 1:20,000
Western Blot : 1:250 - 1:1,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

0.95 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

MFAP5

MAGP-2 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. MAGP-2 (also known as Microfibril-associated glycoprotein 2, MFAP-2, MP25 and Microfibrillar-associated protein 5 precursor) is a secreted, extracellular matrix protein which is a component of the elastin-associated microfibrils. It forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. MAGP-2 is upregulated in some ovarian cancers and is associated with poor survival of ovarian cancer patients.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human MAGP-2 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20-30 kDa in size corresponding to MAGP-2 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity-purified antibody is directed against human MAGP-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with MAGP-2 protein from human and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with MAGP-2 from mouse, dog and rat sources based on partial (~75%) sequence homology. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Gibson,M.A., Hatzinikolas,G., Kumaratilake,J.S., Sandberg,L.B., Nicholl,J.K., Sutherland,G.R. and Cleary,E.G. (1996) Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25). J. Biol. Chem. 271 (2), 1096-1103. Hatzinikolas,G. and Gibson,M.A. (1998) The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region. J. Biol. Chem. 273 (45), 29309-29314. Nehring LC, Miyamoto A, Hein PW, Weinmaster G, Shipley JM. (2005) The extracellular matrix protein MAGP-2 interacts with Jagged1 and induces its shedding from the cell surface. J Biol Chem. 280(21):20349-55. Hanssen E, Hew FH, Moore E, Gibson MA. MAGP-2 has multiple binding regions on fibrillins and has covalent periodic association with fibrillin-containing microfibrils. (2004) J Biol Chem. 279(28):29185-94. Penner AS, Rock MJ, Kielty CM, Shipley JM. (2002) Microfibril-associated glycoprotein-2 interacts with fibrillin-1 and fibrillin-2 suggesting a role for MAGP-2 in elastic fiber assembly. J Biol Chem. 277(38):35044-9. Gibson,M.A., Hatzinikolas,G., Kumaratilake,J.S., Sandberg,L.B., Nicholl,J.K., Sutherland,G.R. and Cleary,E.G. (1996) Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25). J. Biol. Chem. 271 (2), 1096-1103.

Specific Reference

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