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Primary Antibodies  >  Cancer Research Antibodies
 

Cas5 Antibody

Rabbit Polyclonal
Code

600-401-948
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western blot using Rockland's affinity purified anti-hCas5 antibody shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead).  Lane 1 contains a lysate from AS cells transfected with empty vector.   Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa).  Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA  (as tested by Northern blot).  Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA.  Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication.  Carol Thiele, NCI, Bethesda, MD.
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Synonyms: Apoptosis related cysteine protease antibody, apoptosis-related cysteine protease antibody, CASP5 antibody, ICE(rel)III antibody

Cas5 Antibody Properties

Description
Anti-Cas5 (RABBIT) Antibody - 600-401-948
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, chimpanzee, bovine, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.3 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
GeneName
hCAS5
Download CofA
Lot#  

Cas5 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. hCas5 (Castor) is the human homolog of a Drosophila gene that regulates neurogenesis. In human neuro-blastoma tumors where a loss of heterozygosity of CASZ1 is noted, hCas functions to suppress the tumorigenicity of cells. hCas5 is a developmentally regulated transcription factor found in neural and muscle cells. hCas5 is a Zn finger transcription factor that is predominantly found in the nucleus. This antibody is designed to facilitate the functional study of this protein and its role in developmental regulation and expression in human tumor tissues.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against human Cas5 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Cas5 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, Drosophila, chimpanzee, and macaque
Catalog Number
600-401-B62

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, Drosophila, chimpanzee, and macaque
Catalog Number
600-401-B62S

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry