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Anti-Rab11 Family-Interacting Protein 3 (FIP3) (RABBIT) Antibody - 600-401-994

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Primary Antibodies > Cancer Research Antibodies

Rab11 FIB3 Antibody

Rabbit Polyclonal

Code

600-401-994

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Synonyms: IkB kinase associated protein 1 antibody, IkB kinase subunit gamma antibody, Inhibitor of nuclear factor kappa B kinase subunit gamma antibody, AMCBX1 antibody, FIP 3 antibody

Datasheet
References
Customer Reviews
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Rab11 FIB3 Antibody Properties

Description

Anti-Rab11 Family-Interacting Protein 3 (FIP3) (RABBIT) Antibody - 600-401-994

Host

Rabbit

Target Species

Mouse

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:2,000 - 1:10,000
Western Blot : 1:200 - 1:2,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.19 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

RAB11FIP3

Rab11 FIB3 Antibody Description

Background

Rab11 Family-Interacting Protein 3 (FIP3)/Arfophilin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and the ADP-ribosylation factor Arf6. Rab11 and Arf6 are involved in membrane delivery from recycling endosomes to the plasma membrane during cytokinesis. The dual Rab11/Arf6 binding proteins, FIP3 and FIP4, function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis. An interaction between Rab11-FIP3 and ASAP1 has also been identified (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD). ASAP1 is an Arf GTPase-activating protein that has been implicated in the invasive properties of uveal melanoma and mammary carcinoma.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids spanning the carboxyl terminal one-third of human FIP3.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Although the predicted molecular weight of FIP3 is 82.4 kDa, a band corresponding to FIP3 was observed at 120 kDa in human cell lysates and at 150 kDa in mouse cell lysates. The identity of a 40 kDa band, observed in western blotting against both HeLa and 293T cell lysates, is unknown (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD).

Purity/Specifity

This affinity purified antibody is directed against human FIP3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with UPLC1/ASAP3 protein from mouse and rat based on approximately 92% protein:protein homology over the region of the immunizing sequence. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Shiba, T., Koga, H., Shin, H.W., Kawasaki, M., Kato, R., Nakayama, K., Wakatsuki, S. (2006) Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1. Proc. Natl. Acad. Sci. USA 103(42):15416-15421. Fielding, A.B., Schonteich, E., Matheson, J., Wilson, G., Yu, X., Hickson, G.R., Srivastava, S., Baldwin, S.A., Prekeris, R., Gould, G.W. (2005) Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis. EMBO J. 24(19):3389-3399. Prekeris, R., Davies, J.M., Scheller, R.H. (2001) Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins. J. Biol. Chem. 276(42):38966-38970. Randazzo, P.A., Andrade, J., Miura, K., Brown, M.T., Long, Y.Q., Stauffer, S., Roller, P., Cooper, J.A. (2000) The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton. Proc. Natl. Acad. Sci. USA 97(8):4011-4016.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra