14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-MAPKAP Kinase 2 (RABBIT) Antibody - 600-401-281
Anti-MAPKAP Kinase 2 (RABBIT) Antibody - 600-401-281
Anti-GSK3 beta pS9 (RABBIT) Antibody - 600-401-424
Anti-GSK3 beta pS9 (RABBIT) Antibody - 600-401-424
Anti-GSK3 alpha pS21 (RABBIT) Antibody - 600-401-429
Anti-GSK3 alpha pS21 (RABBIT) Antibody - 600-401-429
Anti-GSK3 alpha (RABBIT) Antibody - 600-401-444
Anti-GSK3 alpha (RABBIT) Antibody - 600-401-444
Primary Antibodies  >  Sample Size Primary Antibodies
 

Cripto-1 TDGF1 Antibody

Rabbit Polyclonal
Code

600-401-997S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot using Rockland's affinity purified anti-Cripto-1 antibody shows detection of endogenous and transfected Cripto-1 from mouse and human sources.  The Cripto-1 band appears above the 17.8 kDa marker.  Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression.  COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control.  A non-specific band at 45kDa may be present in some preparations.  The primary antibody was used at a 1:500 dilution.  Personal communication, C. Bianco, NCI, Bethesda, MD
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Cripto1 antibody, Cripto1 growth factor antibody, Epidermal growth factor like cripto protein CR1 antibody, TDGF 1 antibody

Cripto-1 TDGF1 Antibody Properties

Description
Anti-Cripto-1 (TDGF1) (RABBIT) Antibody - 600-401-997S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:50,000 - 1:150,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.80 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
TDGF1
Download CofA
Lot#  

Cripto-1 TDGF1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis.  During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor b (TGFb) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment.  In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation.  Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 21 kDa in size corresponding to Cripto-1 by western blotting in the appropriate cell lysate or extract.  Cripto-1 is reported as a 188 amino acid protein with a molecular weight of 21.1 kDa.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human Cripto-1 protein.  A BLAST analysis was used to suggest limited cross-reactivity with Cripto-1 from mouse based on a 70% homology with the immunizing sequence.  Expect cross-reactivity with human Cripto-3 (TDGF2) based on very high levels of sequence conservation.  Cross-reactivity with Cripto-1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bianco C, Strizzi L, Normanno N, Khan N, Salomon DS  (2005) Cripto-1: an oncofetal gene with many faces. Curr. Top. Dev. Biol. 67:85-133. Bianco C, Strizzi L, Ebert A, Chang C, Rehman A, Normanno N, Guedez L, Salloum R, Ginsburg E, Sun Y, Khan N, Hirota M, Wallace-Jones B, Wechselberger C, Vonderhaar BK, Tosato G, Stetler-Stevenson WG, Sanicola M, Salomon DS (2005) Role of human cripto-1 in tumor angiogenesis. J. Natl Cancer Inst. 97(2):132-141.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-424

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-429

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-444

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry