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Primary Antibodies  >  Sample Size Primary Antibodies
 

Cybr Antibody

Rabbit Polyclonal
Code

600-401-A23S
Size

25 µL
Price

$95.00
Availability

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Western blot using Rockland's affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3).  Lane 1 shows reactivity of pre-immune sera.  Lane 2 shows endogenous Cybr detected with antibody.  Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct western blot of wt splenocytes.  Lane 6 shows direct western blot of knock out mouse.   Personal Communication, V. Coppola, NCI, Bethesda, MD.
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Synonyms: Cytohesin-interacting protein Cytohesin-binding protein HE Cbp Pleckstrin homology Sec7 and coiled-coil domains-binding protein Pscdbp

Cybr Antibody Properties

Description
Anti-Cybr (RABBIT) Antibody - 600-401-A23S
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:1000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.86 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CYTIP
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Lot#  

Cybr Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the carboxy terminal portion of mouse Cybr.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This antibody has been tested for use in ELISA, western blot, and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting in the appropriate cell lysate or extract.  Thisantibody detects endogenous and over-expressed Cybr protein and is capable of immunoprecipitating the antigen from lysates.
Purity/Specifity
This affinity purified antibody is directed against mouse Cybr.  A BLAST analysis was used to suggest cross-reactivity with Cybr from mouse and rat based on a 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Coppola,V., Barrick,C.A., Bobisse,S., Rodriguez-Galan,M.C., Pivetta,M., Reynolds,D., Howard,O.M., Palko,M.E., Esteban,P.F., Young,H.A., Rosato,A. and Tessarollo,L. (2006) The scaffold protein Cybr is required for cytokine-modulated trafficking of leukocytes in vivo. Mol. Cell. Biol. 26 (14), 5249-5258. Watford,W.T., Li,D., Agnello,D., Durant,L., Yamaoka,K., Yao,Z.J., Ahn,H.J., Cheng,T.P., Hofmann,S.R., Cogliati,T., Chen,A., Hissong,B.D., Husa,M.R., Schwartzberg,P., O'Shea,J.J. and Gadina,M. (2006) Cytohesin binder and regulator (cybr) is not essential for T- and dendritic-cell activation and differentiation. Mol. Cell. Biol. 26 (17), 6623-6632. Boehm,T., Hofer,S., Winklehner,P., Kellersch,B., Geiger,C., Trockenbacher,A., Neyer,S., Fiegl,H., Ebner,S., Ivarsson,L., Schneider,R., Kremmer,E., Heufler,C. and Kolanus,W. (2003) Attenuation of cell adhesion in lymphocytes is regulated by CYTIP, a protein which mediates signal complex sequestration. EMBO J. 22 (5), 1014-1024.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-301

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B31

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B32

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry