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Primary Antibodies  >  Cancer Research Antibodies
 

PBK1 Antibody

Rabbit Polyclonal
Code

600-401-A24
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1.  In the overlay, specific antibody staining is shown to co-localize with recombinant protein.  Cells were fixed with methanol prior to staining.      Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Western blot using Rockland's affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1.  Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells.  Lanes 2 and 4 contain lysate from cells transfected with null vector.  Lanes 1 and 2 were blotted with anti-Flag antibody.  Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1.  The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1.  Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
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Synonyms: CATX11 antibody, Cellular senescence inhibited gene protein antibody, CSIG antibody, L12 antibody, Ribosomal L1 domain containing 1 antibody

PBK1 Antibody Properties

Description
Anti-PBK1 (RABBIT) Antibody - 600-401-A24
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,500 - 1:10,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RSL1D1
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Lot#  

PBK1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004).  A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 75 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract.  This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PBK1 protein.  A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence.  Cross-reactivity with PBK1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Huch, G., Hohn, H.P. and Denker, H.W. (1998) Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR. Placenta 19 (8), 557-567. Petroziello, J., Yamane, A., Westendorf, L., Thompson, M., McDonagh, C., Cerveny, C., Law, C.L., Wahl, A. and Carter, P. (2004) Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer. Oncogene 23 (46), 7734-7745. Tong, C., Tan, L., Li, P. and Zhu, Y.S. (2005) Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells. Acta Biochim. Biophys. Sin (Shanghai) 37 (5), 303-309.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry