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Primary Antibodies > Cancer Research Antibodies

PBK1 Antibody

Rabbit Polyclonal

Code

600-401-A24

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

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Synonyms: CATX11 antibody, Cellular senescence inhibited gene protein antibody, CSIG antibody, L12 antibody, Ribosomal L1 domain containing 1 antibody

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PBK1 Antibody Properties

Description

Anti-PBK1 (RABBIT) Antibody - 600-401-A24

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:2,500 - 1:10,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.1 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

RSL1D1

PBK1 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004). A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PBK1 protein. A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence. Cross-reactivity with PBK1 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Huch, G., Hohn, H.P. and Denker, H.W. (1998) Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR. Placenta 19 (8), 557-567. Petroziello, J., Yamane, A., Westendorf, L., Thompson, M., McDonagh, C., Cerveny, C., Law, C.L., Wahl, A. and Carter, P. (2004) Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer. Oncogene 23 (46), 7734-7745. Tong, C., Tan, L., Li, P. and Zhu, Y.S. (2005) Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells. Acta Biochim. Biophys. Sin (Shanghai) 37 (5), 303-309.

Specific Reference

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Antigen Retrieval Methods

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Beta Galactosidase Conjugates

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Biotin Conjugates

Cell Lysates Protocol

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