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Primary Antibodies  >  Stem Cell Antibodies
 

Wnt1 Antibody

Rabbit Polyclonal
Code

600-401-A37
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1.  After transfer, the membrane was blocked with 5% BLOTTO.  Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO.  The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).
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Synonyms: INT1 antibody, Murine mammary tumor virus integration site 1 antibody, Oncogene INT1 antibody, Proto oncogene protein Wnt 1 antibody, Wingless type MMTV integration site family member 1 antibody

Wnt1 Antibody Properties

Description
Anti-Wnt1 (RABBIT) Antibody - 600-401-A37
Host
Rabbit
Target Species
Human
Known Cross Reactivity
mouse, human, rat, bovine, dog, macaque, opossum
Clonality
Polyclonal
Application
ELISA : 1:25,000
Western Blot : 1:1,500 - 1:6,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.38 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
WNT1
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Lot#  

Wnt1 Antibody Description

Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Wnt1 protein.  A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence.  Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology.  Cross-reactivity with Wnt1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
De Ferrari,G.V. and Moon,R.T. (2006) The ups and downs of Wnt signaling in prevalent neurological disorders. Oncogene 25 (57), 7545-7553. Yook,J.I., Li,X.Y., Ota,I., Hu,C., Kim,H.S., Kim,N.H., Cha,S.Y., Ryu,J.K., Choi,Y.J., Kim,J., Fearon,E.R. and Weiss,S.J. (2006) A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells.  Nat. Cell Biol. 8 (12), 1398-1406. He,J., Sheng,T., Stelter,A.A., Li,C., Zhang,X., Sinha,M., Luxon,B.A. and Xie,J.  (2006) Suppressing Wnt signaling by the hedgehog pathway through sFRP-1.  J. Biol. Chem. 281 (47), 35598-35602. Tell,S., Yi,H., Jockovich,M.E., Murray,T.G. and Hackam,A.S. (2006) The Wnt signaling pathway has tumor suppressor properties in retinoblastoma.  Biochem. Biophys. Res. Commun. 349 (1), 261-269. Sievers,S., Fritzsch,C., Grzegorczyk,M., Kuhnen,C. and Muller,O.  (2006) Absolute beta-catenin concentrations in Wnt pathway-stimulated and non-stimulated cells.  Biomarkers 11 (3), 270-278.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, human
Catalog Number
100-401-223

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-424

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-429

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry