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Primary Antibodies  >  Sample Size Primary Antibodies
 

Fibroblast Activation Protein Antibody

Rabbit Polyclonal
Code

600-401-A73S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.  Personal communication, S.Kim, NCI, Bethesda, MD.
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Synonyms: 170 kDa melanoma membrane bound gelatinase antibody, DPPIV antibody, Integral membrane serine protease antibody, FAPA antibody, Seprase antibody

Fibroblast Activation Protein Antibody Properties

Description
Anti-Fibroblast Activation Protein (FAP) (RABBIT) Antibody - 600-401-A73S
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:100,000
Western Blot : 1:500 to 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.81 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
FAP
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Lot#  

Fibroblast Activation Protein Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP)  is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules.  FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4.  Multiple isoforms of FAP are reported as alternative splicing products from a common gene.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody is known to react with mouse FAP protein.  A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence.    Reactivity with FAP from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Cheng,J.D., Valianou,M., Canutescu,A.A., Jaffe,E.K., Lee,H.O., Wang,H., Lai,J.H., Bachovchin,W.W. and Weiner,L.M. (2005) Abrogation of fibroblast activation protein enzymatic activity attenuates tumor growth.  Mol. Cancer Ther. 4 (3), 351-360. Ramirez-Montagut,T., Blachere,N.E., Sviderskaya,E.V., Bennett,D.C., Rettig,W.J., Garin-Chesa,P. and Houghton,A.N. (2004) FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor.  Oncogene 23 (32), 5435-5446. Niedermeyer,J., Garin-Chesa,P., Kriz,M., Hilberg,F., Mueller,E., Bamberger,U., Rettig,W.J. and Schnapp,A. (2001) Expression of the fibroblast activation protein during mouse embryo development.  Int. J. Dev. Biol. 45 (2), 445-447.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-001-271

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-306

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-321

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-264

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry