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Primary Antibodies  >  Cancer Research Antibodies
 

Protein Kinase C Beta Antibody

Rabbit Polyclonal
Code

600-401-A76
Size

100 µg
Price

$309.00
Availability

Ships within 2 weeks.
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Western blot using Rockland's affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 ?g of U251 whole cell lysate (glioma derived).  A 4-12% gel was used for separation.   The arrowhead corresponds to 76 kDa PKC beta.  The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4° C, overnight.  Personal Communication, Howard Fine and Svetlana Kotliarova, CCR-NCI, Bethesda, MD.
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Synonyms: PKC beta

Protein Kinase C Beta Antibody Properties

Description
Anti-Protein Kinase C beta (RABBIT) Antibody - 600-401-A76
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, monkey
Clonality
Polyclonal
Application
ELISA : 1:75,000 - 1:80,000
Western Blot : 1:1,000 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PRKCB
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Lot#  

Protein Kinase C Beta Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis.  PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption.  Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract.  Lysates from U251or BxPC3 cells are suggested for western blotting.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PKC beta protein.  A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence.  Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources.  Cross-reactivity with PKC beta from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Graff JR, McNulty AM, Hanna KR, Konicek BW, Lynch RL, Bailey SN, Banks C, Capen A, Goode R, Lewis JE, Sams L, Huss KL, Campbell RM, Iversen PW, Neubauer BL, Brown TJ, Musib L, Geeganage S, Thornton D.  (2005) The protein kinase Cbeta-selective inhibitor, Enzastaurin (LY317615.HCl), suppresses signaling through the AKT pathway, induces apoptosis, and suppresses growth of human colon cancer and glioblastoma xenografts.  Cancer Res. 65(16):7462-9. Koivunen J, Aaltonen V, Koskela S, Lehenkari P, Laato M, Peltonen J. (2004) Protein kinase C alpha/beta inhibitor Go6976 promotes formation of cell junctions and inhibits invasion of urinary bladder carcinoma cells.  Cancer Res. 64(16):5693-701. Zhang J, Wang L, Petrin J, Bishop WR, Bond RW.(1993)  Characterization of site-specific mutants altered at protein kinase C beta 1 isozyme autophosphorylation sites. Proc Natl Acad Sci U S A. 90(13):6130-4.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry