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Primary Antibodies > Sample Size Primary Antibodies

MLF1 Antibody

Rabbit Polyclonal

Code

600-401-A91S

Size

25 µL

Price

$89.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-MLF1IP antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells treated with control luciferase shRNA (lane 1), and detection of MLF1IP in Hela cells transfected with MLF1IP (lane 3). Lane 2: Hela cells treated with MLF1IP shRNA. The identity of the lower molecular weight bands is unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.

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Synonyms: HHV8 LNAIP1 antibody, ICEN24 antibody, Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1 antibody, KLIP-1 antibody, KLIP1 antibody, KSHV latent nuclear antigen interacting protein 1 antibody

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MLF1 Antibody Properties

Description

Anti-MLF1 Interacting Protein (C-terminal specific) (RABBIT) Antibody - 600-401-A91S

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:650,000
Western Blot : 1:500- 1:2,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

MLF1IP

MLF1 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 418 residue recombinant protein corresponding to the carboxy terminal end of human MLF1IP protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against human MLF1IP protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Hanissian,S.H., Teng,B., Akbar,U., Janjetovic,Z., Zhou,Q., Duntsch,C. and Robertson,J.H. (2005) Regulation of myeloid leukemia factor-1 interacting protein (MLF1IP) expression in glioblastoma. Brain Res. 1047 (1), 56-64. Hanissian,S.H., Akbar,U., Teng,B., Janjetovic,Z., Hoffmann,A., Hitzler,J.K., Iscove,N., Hamre,K., Du,X., Tong,Y., Mukatira,S., Robertson,J.H. and Morris,S.W. (2004) cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP. Oncogene 23 (20), 3700-3707. Pan,H.Y., Zhang,Y.J., Wang,X.P., Deng,J.H., Zhou,F.C. and Gao,S.J. (2003) Identification of a novel cellular transcriptional repressor interacting with the latent nuclear antigen of Kaposi's sarcoma-associated herpesvirus. J. Virol. 77 (18), 9758-9768.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra