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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Mer2 phospho S30 Antibody

Rabbit Polyclonal
Code

600-401-924
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts.  Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis.  Proteins were obtained using TCA precipitation.  The primary antibody was used at a 1:7,500 dilution.  Secondary antibody was used at 1:5,000 dilution.  Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Western blot using Rockland's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells.  Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody.  Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2.  Lane 2 contains the same preparation after treatment with phosphatase.  Lane 3 contains Mer2-S30A protein as a phosphorylation control.  This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphor-ylated protein.  The primary antibody was used at a 1:5,000 dilution.  Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
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Synonyms: Meiotic recombination 2 protein REC107

Mer2 phospho S30 Antibody Properties

Description
Anti-Mer2 pS30 (RABBIT) Antibody - 600-401-924
Host
Rabbit
Target Species
Saccharomyces cerevisiae
Known Cross Reactivity
S.cerevisiae
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:1,000 - 1:10,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MER2, REC107
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Mer2 phospho S30 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression.  It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog.  This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases.   Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with the replication fork.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract.  Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue.  Preparation of extracts from cells 4hr after initiation of meiosis is suggested.
Purity/Specifity
This affinity-purified antibody is directed against the phosphorylated form of Saccharomyces cerevisiae Mer2 protein at the pS30 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Engebrecht,J., Hirsch,J. and Roeder,G.S. (1990) Meiotic gene conversion and crossing over: their relationship to each other and to chromosome synapsis and segregation. Cell 62 (5), 927-937. Engebrecht,J.A., Voelkel-Meiman,K. and Roeder,G.S. (1991) Meiosis-specific RNA splicing in yeast. Cell 66 (6), 1257-1268. Hani,J., Stumpf,G. and Domdey,H. (1995) PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows strong homology with a new putative family of PPIases. FEBS Lett. 365 (2-3), 198-202.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
211-1202

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
S.cerevisiae
Catalog Number
600-401-925

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry