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Primary Antibodies > Cell Cycle Protein Antibodies

Cyclin E2 Antibody

Rabbit Polyclonal

Code

600-401-971

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

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Synonyms: G1/S-specific cyclin-E2

Datasheet
References
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Cyclin E2 Antibody Properties

Description

Anti-Cyclin E2 (RABBIT) Antibody - 600-401-971

Host

Rabbit

Target Species

Human

Known Cross Reactivity

mouse, human

Clonality

Polyclonal

Application

ELISA : 1:20,000 - 1:85,000
Western Blot : 1:200 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.17 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

CCNE2

Cyclin E2 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions. Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against mouse Cyclin E2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Cyclin E2 protein from human based on 100% homology with the immunizing sequence. Cross-reactivity with Cyclin E2 from rat is also predicted based on a 91% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Philipp, G.Y., Yu, Q., Sicinska, E., Das, M., Schneider, J.E., Bhattacharya, S., Rideout, W.M., Bronson, R.T., Gardner, H., Sicinski, P. (2003) Cyclin E ablation in the mouse. Cell 114(4):431-443. Yu, Q., Sicinski, P. (2004) Mammalian cell cycles without Cyclin E-CDK2. Cell Cycle 3(3):292-295. Parisi, T., Beck, A.R., Rougier, N., McNeil, T., Lucian, L., Werb, Z., Amati, B. (2003) Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells. EMBO J. 22(18):4794-4803. Geng, Y., Yu, Q., Whoriskey, W., Dick, F., Tsai, K.Y., Ford, H.L., Biswas, D.K., Pardee, A.B., Amati, B., Jacks, T., Richardson, A., Dyson, N., Sicinski, P. (2001) Expression of Cyclins E1 and E2 during mouse development and in neoplasia. Proc. Natl. Acad. Sci. USA 98(23):13138-13143. Berthet, C., Aleem, E., Coppola, V., Tessarollo, L., Kaldis, P. (2003) Cdk2 knockout mice are viable. Curr. Biol. 13(20):1775-1785. Aleem, E., Kiyokawa, H., Kaldis, P. (2005) Cdc2-Cyclin E complexes regulate the G1/S phase transition. Nat. Cell Biol. 7(8):831-836.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra