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Primary Antibodies > Cytokines & Growth Factor Antibodies

Angiopoietin 1 Antibody

Rabbit Polyclonal

Code

100-401-403

Size

200 µL

Price

$294.00

Availability

Ships next business day.

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Rabbit anti-Ang-1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecip-itation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development.

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Synonyms: AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, ANG1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody

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Angiopoietin 1 Antibody Properties

Description

Anti-Angiopoietin 1 (RABBIT) Antibody - 100-401-403

Host

Rabbit

Target Species

Mouse

Known Cross Reactivity

human, mouse

Clonality

Polyclonal

Application

ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500-1:2,000
Immunohistochemistry: 1:200-1:800
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

Antiserum

Label

Unconjugated

Concentration

85 mg/mL by Refractometry

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

ANG1

Angiopoietin 1 Antibody Description

Background

Anti Antiopoietin-1 Antibody recognizes Angiopoietin-1 (Ang-1) that has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Suitable for western blotting, Immunohistochemistry (IHC) and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.

Purity/Specifity

This antiserum is directed against mouse angiopoietin 1 and shows no reactivity with mouse angiopoietin 2. A 57 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Suri, C., Jones, P.F., Patan, S., Bartunkova, S., Maisonpierre, P.C., Davis, S., Sato, T.N. and Yancopoulos, G.D. (1996) Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell, 87(7): 1171-1180. Davis, S., Aldrich, T.H., Jones, P.F., Acheson, A., Compton, D.L., Jain, V., Ryan, T.E., Bruno, J., Radziejewski, C., Maisonpierre, P.C. and Yancopoulos, G.D. (1996) Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell, 87(7): 1161-1169. Maisonpierre, P.C., Suri, C., Jones, P.F., Bartunkova, S., Wiegand, S.J., Radziejewski, C., Compton, D., McClain, J., Aldrich, T.H., Papadopoulos, N., Daly, T.J., Davis, S., Sato, T.N. and Yancopoulos, G.D. (1997) Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science, 277(5322): 55-60.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra