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Primary Antibodies  >  Stem Cell Antibodies
 

Leptin Antibody

Rabbit Polyclonal
Code

110-401-320
Size

1 mL
Price

$309.00
Availability

Ships next business day.
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Synonyms: LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

Leptin Antibody Properties

Description
Anti-Leptin (RABBIT) Antibody - 110-401-320
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Leptin
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Lot#  

Leptin Antibody Description

Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum against Mouse Leptin has been tested for use in ELISA and immunoblotting.  Reactivity in other immunoassays is unknown.  Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes.This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,400 is suggested for this product. 
Purity/Specifity
This antiserum has been heated to 56°C for 30 minutes.  The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.  Reactivity with leptin from other sources is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-320

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-320

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
100-401-861

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry