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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-7 Receptor Alpha Chain Antibody

Rabbit Polyclonal
Code

600-401-A48
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Dot Blot of Rabbit anti-IL-7 Receptor antibody.  
Antigen:  IL-7 Receptor and IL-7 Receptor pY449 forms of the immunizing peptide and THRB1 and THRB1 pY171 as controls.
Load: 3.0, 0.3, 0.1, 0.05 µg as indicated.
Primary antibody:  IL-7 Receptor antibody at 1:400 for 45 min at 4°C.
Secondary antibody:  Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: IL7R alpha antibody, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor antibody

IL-7 Receptor Alpha Chain Antibody Properties

Description
Anti-IL-7 Receptor alpha chain (RABBIT) Antibody - 600-401-A48
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000-1:50,000
Western Blot : 1:1,000 - 1:10,000
Immunochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.82 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL17R
Download CofA
Lot#  

IL-7 Receptor Alpha Chain Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.  The human and mouse sequence is nearly identical.  In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain Antibody is ideal for investigators interested in Immunology research.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 receptor protein.
Immunogen Type
Peptide
Storage Condition
Store IL-7 Receptor Alpha Chain Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to IL-7 receptor protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads.  This antibody is specific for mouse IL-7 receptor protein.  A BLAST analysis was used to suggest cross-reactivity with IL-7 receptor protein from human, mouse and rat sources based on 100% homology with the immunizing sequence.  Cross-reactivity with IL-7 receptor protein from other sources has not been determined. Immunology Research
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Osborne,L.C., Dhanji,S., Snow,J.W., Priatel,J.J., Ma,M.C., Miners,M.J., Teh,H.S., Goldsmith,M.A. and Abraham,N. (2007) Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice.  J. Exp. Med. 204 (3), 619-631. Hartigan-O'Connor,D.J., Poon,C., Sinclair,E. and McCune,J.M. (2007) Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells.  J. Immunol. Methods 319 (1-2), 41-52. Castellino,F. and Germain,R.N.  (2007) Chemokine-guided CD4+ T cell help enhances generation of IL-6RalphahighIL-7Ralpha high prememory CD8+ T cells.  J. Immunol. 178 (2), 778-787. Jiang,Q., Huang,J., Li,W.Q., Cavinato,T., Keller,J.R. and Durum,S.K. (2007) Role of the intracellular domain of IL-7 receptor in T cell development.  J. Immunol. 178 (1), 228-234. Shklovskaya,E. and Fazekas de St Groth,B.  (2006) Severely impaired clonal deletion of CD4+ T cells in low-dose irradiated mice: role of T cell antigen receptor and IL-7 receptor signals.  (2006) J. Immunol. 177 (12), 8320-8330. He,Y.W., Adkins,B., Furse,R.K. and Malek,T.R.  (1995) Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor.  J. Immunol. 154 (4), 1596-1605. Venkitaraman,A.R. and Cowling,R.J. (1994) Interleukin-7 induces the association of phosphatidylinositol 3-kinase with the alpha chain of the interleukin-7 receptor.  Eur. J. Immunol. 24 (9), 2168-2174.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B94

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B94

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-403-B94

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-406-B94

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry