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IL-7 Receptor Alpha Chain phospho Y449 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody.  
Lane 1:  thymocyte D1 cells.
Lane 2:  thymocyte D1 cells treated with IL7 (50 ng/ml).
IP: with anti-Phosphotyrosine conjugated to Protein G agarose.
Primary antibody:  IL7pY449 antibody at 1:10000 for overnight at 4°C.
Secondary antibody:  HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  55 kDa, ~64 kDa for IL7pY449. 
Other band(s): unspecifics.
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100 µg
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IL-7 Receptor Alpha Chain phospho Y449 Antibody Properties

Anti-IL-7 Receptor alpha chain pY449 (RABBIT) Antibody - 600-401-A49
Target Species
Known Cross Reactivity
human, mouse, rat
ELISA : 1:5,000-1:50,000
Western Blot : 1:10,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
0.82 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
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IL-7 Receptor Alpha Chain phospho Y449 Antibody Description

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.  The human and mouse sequence is nearly identical.  In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.
IL7Ralpha antibody, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor antibody
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Immunogen Type
Post Translational Modification
Storage Condition
Store IL-7 Receptor Alpha Chain phospho Y449 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against non-phospho-peptide. This antibody is specific for mouse IL-7 protein phosphorylated at Y449.  A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence.  Cross-reactivity with IL-7 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Osborne,L.C., Dhanji,S., Snow,J.W., Priatel,J.J., Ma,M.C., Miners,M.J., Teh,H.S., Goldsmith,M.A. and Abraham,N. (2007) Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice.  J. Exp. Med. 204 (3), 619-631. Hartigan-O'Connor,D.J., Poon,C., Sinclair,E. and McCune,J.M. (2007) Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells.  J. Immunol. Methods 319 (1-2), 41-52. Castellino,F. and Germain,R.N.  (2007) Chemokine-guided CD4+ T cell help enhances generation of IL-6RalphahighIL-7Ralpha high prememory CD8+ T cells.  J. Immunol. 178 (2), 778-787. Jiang,Q., Huang,J., Li,W.Q., Cavinato,T., Keller,J.R. and Durum,S.K. (2007) Role of the intracellular domain of IL-7 receptor in T cell development.  J. Immunol. 178 (1), 228-234. Shklovskaya,E. and Fazekas de St Groth,B.  (2006) Severely impaired clonal deletion of CD4+ T cells in low-dose irradiated mice: role of T cell antigen receptor and IL-7 receptor signals.  (2006) J. Immunol. 177 (12), 8320-8330. He,Y.W., Adkins,B., Furse,R.K. and Malek,T.R.  (1995) Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor.  J. Immunol. 154 (4), 1596-1605. Venkitaraman,A.R. and Cowling,R.J. (1994) Interleukin-7 induces the association of phosphatidylinositol 3-kinase with the alpha chain of the interleukin-7 receptor.  Eur. J. Immunol. 24 (9), 2168-2174.
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