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Primary Antibodies  >  DNA Damage and Repair Antibodies

MCM2 Antibody

Rabbit Polyclonal


100 µg


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Western blot using Rockland's Affinity Purified anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 present in nuclear extracts from elutriated human cells (MO59K/K562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the protein is unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase.  In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (lane 3).  Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations.   Asynchronous cells contain a doublet of both forms.  Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate.   The phosphorylated band migrates faster than the unphosphorylated form and is seen as the lower band.  There is a clear switch from the unphosphorylated form in the center lane, to the phosphorylated form in the third lane, confirming recognition of both forms of MCM2 by this antibody.  The primary antibody was diluted 1:400 for this experiment.  Personal Communication, Jennifer Seiler, NIH, CCR, Bethesda, MD.
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$20.00 to United States
Synonyms: BM28 antibody, CCNL 1 antibody, CCNL1 antibody, CDC like 1 antibody, Minichromosome maintenance complex component 2 antibody

MCM2 Antibody Properties

Anti-MCM2 (RABBIT) Antibody - 600-401-922
Target Species
Known Cross Reactivity
human, mouse, rat, S. cerevisiae
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
0.98 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
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MCM2 Antibody Description

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. MCM2, also called DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog or Nuclear Protein BM28) is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by protein kinases CDC2 and CDC7.  Double serines at positions 26 and 27 are conserved in rat and mouse.  High homology >88% to similar proteins from several other species.  Expected broad reactivity to various lysates.
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 21-31 of human MCM2 protein (see below).
Immunogen Type
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 100 kDa in size corresponding to phosphorylated or unphosphorylated MCM2 proteins by western blotting in the appropriate cell lysate or extract.
This affinity-purified antibody is directed against human MCM2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.   Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27).  A BLAST analysis was used to suggest cross reactivity with MCM2 proteins from human, mouse, rat and S. cerevisiae based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Shukla,A., Navadgi,V.M., Mallikarjuna,K. and Rao,B.J. (2005) Interaction of hRad51 and hRad52 with MCM complex: a cross-talk between recombination and replication proteins. Biochem. Biophys. Res. Commun. 329 (4), 1240-1245. Tsvetkov,L. and Stern,D.F. (2005) Interaction of chromatin-associated Plk1 and Mcm7. J. Biol. Chem. 280 (12), 11943-11947. Todorov,I.T., Pepperkok,R., Philipova,R.N., Kearsey,S.E., Ansorge,W. and Werner,D. (1994) A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division. J. Cell. Sci. 107 (PT 1), 253-265.
Specific Reference
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Product Type
Primary Antibodies;
Reacts With
human, rat and mouse
Catalog Number

Product Type
Secondary Antibodies;
Catalog Number

Product Type
Primary Antibodies;
Reacts With
human, chimpanzee
Catalog Number

Product Type
Supporting Reagents;
Catalog Number

Product Label


Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry