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Thyroid Hormone Receptor beta 1 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's affinity purified anti-THRB1 antibody shows detection of in vitro translated THRB1 (lane 2) and human thyroid stable cell line expressing THTRb1 (lane 3).  No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA1 (lane 5). The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB.  Personal communication, H. Ying, NCI, Bethesda, MD
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Number:
600-401-A96
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Thyroid Hormone Receptor beta 1 Antibody Properties

Description
Anti-Thyroid Hormone Receptor ß1 (THRB1) (RABBIT) Antibody - 600-401-A96
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:20,000 – 1:200,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
THRB1
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Thyroid Hormone Receptor beta 1 Antibody Description

Background
Anti-Thyroid Hormone Receptor ß1 (TRB1) antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Defects in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). This TRB1 antibody is ideal for Immunology, Thyroid and Signal Tranduction research.
Synonyms
Thyroid hormone receptor beta TRB1 TR beta signaling c-erbA-beta c-erbA-2 Nuclear receptor subfamily 1 group A member 2
Immunogen
Anti-TR ß1 antibody is affinity purified from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.
Immunogen Type
Peptide
Storage Condition
Store Anti-TR beta1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRB protein.  A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence.  Cross-reactivity with THRB from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Cheng, Sheue-yann. (2000) Multiple Mechanisms for Regulations of Transcriptional Activity of Thyroid Hormone Receptors. Reviews in Endocrine & Metabolic Disorders. 1:9-18. Nascimento,A.S., Dias,S.M., Nunes,F.M., Aparicio,R., Ambrosio,A.L., Bleicher,L., Figueira,A.C., Santos,M.A., de Oliveira Neto,M., Fischer,H., Togashi,M., Craievich,A.F., Garratt,R.C., Baxter,J.D., Webb,P. and Polikarpov,I. (2006) Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function. J. Mol. Biol. 360 (3), 586-598. Wan,W., Farboud,B. and Privalsky,M.L. (2005) Pituitary resistance to thyroid hormone syndrome is associated with T3 receptor mutants that selectively impair beta2 isoform function. Mol. Endocrinol. 19 (6), 1529-1542.
Specific Reference
Mascanfroni, Ivan D ; del Mar Montesinos M; Alamino Vanina A. ; Susperreguy S, Nicola JP, Ilarregui JM, Masini-Repiso AM, Rabinovich GA, Pellizas CG (2010) Nuclear Factor (NF)-kappa B-dependent Thyroid Hormone Receptor beta(1) Expression Controls Dendritic Cell Function via Akt Signaling. Journal of Biological Chemistry 285 (13), 9569-9582. DOI: 10.1074/jbc.M109.071241 Published: MAR 26 2010
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
human
Catalog Number
600-401-A38

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
Human
Catalog Number
600-401-A38S

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry