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Primary Antibodies > Enzyme Antibodies

Thyroid Hormone Receptor beta 1 Antibody

Rabbit Polyclonal

Code

600-401-A96

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-THRB1 antibody shows detection of in vitro translated THRB1 (lane 2) and human thyroid stable cell line expressing THTRb1 (lane 3). No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA1 (lane 5). The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB. Personal communication, H. Ying, NCI, Bethesda, MD

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Synonyms: Thyroid hormone receptor beta TRB1 TR beta signaling c-erbA-beta c-erbA-2 Nuclear receptor subfamily 1 group A member 2

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Thyroid Hormone Receptor beta 1 Antibody Properties

Description

Anti-Thyroid Hormone Receptor ß1 (THRB1) (RABBIT) Antibody - 600-401-A96

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse, rat

Clonality

Polyclonal

Application

ELISA : 1:20,000 – 1:200,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

THRB1

Thyroid Hormone Receptor beta 1 Antibody Description

Background

Anti-Thyroid Hormone Receptor ß1 (TRB1) antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Defects in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). This TRB1 antibody is ideal for Immunology, Thyroid and Signal Tranduction research.

Immunogen

Anti-TR ß1 antibody is affinity purified from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.

Immunogen Type

Peptide

Storage Condition

Store Anti-TR beta1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRB protein. A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRB from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Cheng, Sheue-yann. (2000) Multiple Mechanisms for Regulations of Transcriptional Activity of Thyroid Hormone Receptors. Reviews in Endocrine & Metabolic Disorders. 1:9-18. Nascimento,A.S., Dias,S.M., Nunes,F.M., Aparicio,R., Ambrosio,A.L., Bleicher,L., Figueira,A.C., Santos,M.A., de Oliveira Neto,M., Fischer,H., Togashi,M., Craievich,A.F., Garratt,R.C., Baxter,J.D., Webb,P. and Polikarpov,I. (2006) Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function. J. Mol. Biol. 360 (3), 586-598. Wan,W., Farboud,B. and Privalsky,M.L. (2005) Pituitary resistance to thyroid hormone syndrome is associated with T3 receptor mutants that selectively impair beta2 isoform function. Mol. Endocrinol. 19 (6), 1529-1542.

Specific Reference

Mascanfroni, Ivan D ; del Mar Montesinos M; Alamino Vanina A. ; Susperreguy S, Nicola JP, Ilarregui JM, Masini-Repiso AM, Rabinovich GA, Pellizas CG (2010) Nuclear Factor (NF)-kappa B-dependent Thyroid Hormone Receptor beta(1) Expression Controls Dendritic Cell Function via Akt Signaling. Journal of Biological Chemistry 285 (13), 9569-9582. DOI: 10.1074/jbc.M109.071241 Published: MAR 26 2010

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra

ELISA