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Anti-IKKα (RABBIT) Antibody - 100-401-219
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Primary Antibodies  >  Extracellular Matrix Antibodies
 

Osteopontin Antibody

Rabbit Polyclonal
Code

100-401-404
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry.  Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells).  There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Rabbit anti-osteopontin was used at a 1:1,000 dilution to detect Osteopontin by Western blot.  In lane 2, reactivity is shown against 250 ng of human osteopontin.  Lane 3 shows reactivity of MMP-cleaved osteopontin.  Lane 1 shows the position of molecular weight markers.  Use a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) for detection.
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Synonyms: Osteopontin Bone sialoprotein 1 Secreted phosphoprotein 1 SPP-1 Urinary stone protein Nephropontin Uropontin SPP1 BNSP, OPN ORF Name: PSEC0156

Osteopontin Antibody Properties

Description
Anti-Osteopontin (RABBIT) Antibody - 100-401-404
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human,mouse, rat, dog, swine
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:100 - 1:300
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SPP1, BNSP, OPN
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Lot#  

Osteopontin Antibody Description

Background
Anti Osteopontin Antibody recognizes Osteopontin (OPN) that is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein which interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1.  Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN.  OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells.  It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Osteopontin is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections) and ELISA. The antibody exclusively recognizes C-terminal fragments of both thrombin and MMP-cleaved OPN.  A 1:1000 dilution will detect strongly approximately 250 ng of OPN protein on a blot. No pretreatment is required for IHC when formalin-fixed paraffin-embedded tissue is stained.
Purity/Specifity
Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment.  Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Weber, G. F. and Cantor, H. (1996) Cytokine Growth Factor Rev. 7, 241–248. Denhardt, D. T., Lopez, C. A., Rollo, E. E., Hwang, S. M., An, X. R. and Walther, S. E. (1995) Ann. N. Y. Acad. Sci. 760, 127–142. Denhardt, D. T. and Chambers, A. F. (1994) J. Cell. Biochem. 56, 48–51. Uede, T., Katagiri, Y., Iizuka, J. and Murakami, M. (1997) Microbiol. Immunol. 41, 641–648.
Specific Reference
Kim SJ, Shin TK. Immunohistochemical study of osteopontin in boar testis. J Vet Sci. 2007 June; 8(2): 107–110. Published online 2007 June 30. doi: 10.4142/jvs.2007.8.2.107 PMCID: PMC2872706 Robertson BW, Bonsal L, Chellaiah MA. Regulation of Erk1/2 activation by osteopontin in PC3 human prostate cancer cells. Mol. Cancer. 2010;9:260. Robertson BW, Chellaiah MA. Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Exp. Cell Res. 2010 Jan 1;316(1):1–11.
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Product Type
Proteins and Peptides;
Catalog Number
000-000-404

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-C22

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-C22

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-401-219

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry