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Primary Antibodies  >  Kinase and Phosphatase Antibodies
 

TrkCT1 Antibody

Rabbit Polyclonal
Code

600-401-993
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
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Synonyms: ETV6 NTRK3 fusion antibody, GP145 TrkC antibody, gp145(trkC) antibody, GP145TrkC antibody, Neurotrophic tyrosine kinase receptor type 3 antibody, Ntrk3 antibody

TrkCT1 Antibody Properties

Description
Anti-TrkCT1 (RABBIT) Antibody - 600-401-993
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:1,000 - 1:10,000
ImmunoPrecipitation: 1 µg
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.53 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
TRKCT1
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Lot#  

TrkCT1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. TrkCT1, also named neurotrophic tyrosine kinase receptor type 3 (Ntrk3) non-catalytic isoform 2, is a non-catalytic isoform of TrkC, the high affinity receptor for Neurotrophin-3 (NT-3).  This isoform lacks the kinase domain that is responsible for signaling by the full-length isoform.  TrkCT1 is the product of an alternative splicing of Ntrk3 that leaves the extracellular and transmembrane domains intact but includes a shorter intracellular domain encoded by exons 13b and 14b.  Recent studies indicate that the short cytoplasmic tail binds the scaffold protein Tamalin in a ligand-dependent manner and further activates the Arf6–Rac1 signaling pathway (Esteban et al., 2006).
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse TrkCT1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user.  While the predicted molecular weight for TrkCT1 is approximately 70 kDa, the protein has been reported to migrate at about 95 kDa on western blots.  The molecular weight noted for TrkCT1 depends somewhat upon the cell lysate or extract used for western blotting, and this variation may be due to post-translational modifications of the protein.
Purity/Specifity
This affinity purified antibody is directed against mouse TrkCT1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with TrkCT1 protein from human, mouse and rat based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Esteban, P.F., Yoon, H.Y., Becker, J., Dorsey, S.G., Caprari, P., Palko, M.E., Coppola, V., Saragovi, H.U., Randazzo, P.A. and Tessarollo, L. (2006) A kinase-deficient TrkC receptor isoform activates Arf6–Rac1 signaling through the scaffold protein tamalin. J. Cell Biol. 173 (2): 291-299. Palko, M.E., Coppola, V. and Tessarollo, L. (1999) Evidence for a role of truncated trkC receptor isoforms in mouse development. J. Neurosci. 19 (2): 775-782. Menn, B., Timsit, S., Calothy, G. and Lamballe, F. (1998) Differential expression of TrkC catalytic and noncatalytic isoforms suggests that they act independently or in association. J. Comp. Neurol. 401 (1): 47-64.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-C27

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry