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Controls  >  Loading Control Antibodies
 

Alpha-Tubulin Antibody

Mouse Monoclonal 17H11.F10 IgG1 kappa
Code

200-301-880
Size

100 µg
Price

$265.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Rockland's anti-a-tubulin monoclonal antibody was used at a 2.5 µg/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image).  Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.  Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.
HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 µg of lysate each.  The blot was blocked with Blocking Buffer (p/n MB-070) for 30 min at RT, then washed and incubated with Rockland's  anti-Tubulin monoclonal antibody diluted in Blocking Buffer (p/n MB-070) at 1:1,000 for 1 h at RT.   After washing, blot was incubated with a 1:40,000 dilution of Rockland's HRP Rb a-Ms IgG (p/n 610-4302) secondary antibody  in Blocking Buffer (p/n MB-070) for 30 minutes at RT.  Data was collected using Bio-Rad VersaDoc® 4000 MP.
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Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous Tubulin K-alpha-1, TUBA1B, tubulin loading control

Alpha-Tubulin Antibody Properties

Description
Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse, rat. chicken, bovine
Clonality
Monoclonal 17H11.F10 IgG1 kappa
Application
ELISA : 1:300,000
IF Microscopy : 0.1 µg/mL
Western Blot : 1:1,000
Immunochemistry: 2.5 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
TUBA1B
Related Links
Download CofA
Lot#  

Alpha-Tubulin Antibody Description

Background
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
Immunogen
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human alpha tubulin protein.
Storage Condition
Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubliin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Purity/Specifity
Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin.  A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Hall,J.L. and Cowan,N.J. (1985) Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13 (1), 207-223. Cowan,N.J., Dobner,P.R., Fuchs,E.V. and Cleveland,D.W. (1983) Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions. Mol. Cell. Biol. 3 (10), 1738-1745.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
600-401-880

Product Type
Controls;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, chicken, bovine, goat, sheep
Catalog Number
600-403-880

Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, chicken, bovine, goat, sheep
Catalog Number
600-406-880

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry