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Epitope Tag Protein Marker Lysate

NCI Collaboration
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Western blot of 12-Epitope Tag Protein Marker lysate.  Blot was probed with Rockland's affinity purified anti-VSV-G antibody.  The blot shows intense signal at approximately 42 kDa corresponding to the 12-Epitope Tag Protein Marker.  The recommended load is 1 µl of lysate per lane for SDS-PAGE followed by western blotting.  The membrane was probed with primary antibody at a 1:1,000 dilution in 5% BLOTTO in TBST overnight at 4°C.
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Number:
MB-301-0100
Size:
100 µL
Price:
$115.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Epitope Tag Protein Marker Lysate Properties

Description
12 Epitope Tag Protein Marker Lysate - MB-301-0100
Application
Other Dilution: User Optimized
Product Type
MW Standards
Product Type
Reagent
Physical State
Liquid (in 1x Loading Buffer)
Shipping Condition
Dry Ice
Label
Concentration
N/A
Buffer
See application note.
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Epitope Tag Protein Marker Lysate Description

Background
This product is intended as a positive control for use with anti-epitope tag antibodies.  Epitope tags are short peptide sequences easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
Synonyms
Flag, MBP, HA, 6XHIS, Lysate
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.
Application Note
This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer, heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting. The product is supplied in 1X loading buffer and is ready-to-use. 62.5 mM Tris HCl, 10% Glycerol, 2% SDS, 0.05% bromphenol blue, pH 6.8 + 1% Triton 100 + 2.5 mM EDTA+5mM Tris HCl+4% (v/v) ß-Mercaptoethanol.
Purity/Specifity
Loading control for Western blotting. Lysate expresses 12 different epitope tags
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry