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Primary Antibodies  >  Stem Cell Antibodies
 

NOTCH 2 Antibody

Rabbit Polyclonal
Code

100-401-406
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Anti-Notch 2 (intra)  antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2).  Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection.  Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points.  The band at about 110kD represents active Notch 2. This band is not seen in the control cell.  The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band.  Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution.
Western blot using Rockland's anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2).  Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection.  Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points.  The band at about 110kD represents active Notch 2. This band is not seen in the control cell.  The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band.  Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution.
ECL was used as visualization method.
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Synonyms: AGS 2 antibody, AGS2 antibody, hN2 antibody, N2 antibody, Neurogenic locus notch homolog protein 2 antibody, Notch 2 antibody, Notch 2 intracellular domain antibody, Notch homolog 2 antibody

NOTCH 2 Antibody Properties

Description
Anti-NOTCH 2 (intra) (Human specific) (RABBIT) Antibody - 100-401-406
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, dog, chimpanzee
Clonality
Polyclonal
Application
ELISA : 1:30,000 - 1:90,000
Western Blot : 1:400 - 1:2,000
Immunochemistry: 1:200-1:800
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
NOTCH2
Download CofA
Lot#  

NOTCH 2 Antibody Description

Background
Anti Notch 2 Antibody recognizes Notch 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.  Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa).  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA and western blot.  A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC). Specific conditions for reactivity should be optimized by the end user. For western blot experiments, one can expect a band of ˜110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate.
Purity/Specifity
This antiserum is directed against human NOTCH 2.  The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence.  Only one (1) amino acid difference is found in mouse and this change is non-conservative.  Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2.   No specific information is available for other reactivities.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW. (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102. Gridley T,  (2003)  Notch signaling and inherited disease syndromes. Hum Mol Genet: 12,  Suppl 1, R9-R13.
Specific Reference
Gupta S, Li S, Abedin Md J, Wang L, Schneider E, Najafian B, Rosenberg M. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol 298: F209–F215, 2010. Published online before print October 14, 2009, doi: 10.?1152/?ajprenal.?00451.?2009
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
100-401-407

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-408

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
Human, mouse
Catalog Number
200-401-698

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry