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Primary Antibodies  >  Stem Cell Antibodies
 

DELTA-4 Antibody

Rabbit Polyclonal
Code

600-401-696
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Affinity Purified anti-Delta-4 antibody shows detection of a 74-kDa band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue.  Approximately 20 µg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody.  Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.  A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.
Rockland's Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary.  Tissue was formalin-fixed and paraffin embedded.  Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Synonyms: DELTA-4, delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor

DELTA-4 Antibody Properties

Description
Anti-DELTA-4 (RABBIT) Antibody - 600-401-696
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, partial reactivity to mouse and rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 10 µg/mL to 20 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.93 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
DLL4
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Lot#  

DELTA-4 Antibody Description

Background
DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.  DELTA-4  plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.
Immunogen
Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.
Purity/Specifity
DELTA-4 affinity purified antibody is directed against human DELTA-4. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence.  However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence.  Cross reactivity with DELTA-4 homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lauret,E., et al. (2004) Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential. Leukemia 18 (4), 788-797. Tohda,S., Murata-Ohsawa,M., Sakano,S. and Nara,N. (2003) Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int. J. Oncol. 22 (5), 1073-1079. Liu,Z.J., Shirakawa,T., Li,Y., Soma,A., Oka,M., Dotto,G.P., Fairman,R.M., Velazquez,O.C. and Herlyn,M. (2003) Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol. Cell. Biol. 23 (1), 14-25.
Specific Reference
Indraccolo S, Minuzzo S, Masiero M, et al. Cross-talk between tumor and endothelial cells involving the Notch3-Dll4 interaction marks escape from tumor dormancy. Cancer Res. 2009 Feb 15;69(4):1314–1323. Wu S, Esumi S, Watanabe K, et al. Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon. Development. 2011 Jun;138(12):2499–2509. Zhang Z, Zeng B, Zhang Z, et al. Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression. Cancer Res. 2009 Feb 15;69(4):1578–1586. Lauret,E., et al. (2004) Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential. Leukemia 18 (4), 788-797. Tohda,S., Murata-Ohsawa,M., Sakano,S. and Nara,N. (2003) Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int. J. Oncol. 22 (5), 1073-1079. Liu,Z.J., Shirakawa,T., Li,Y., Soma,A., Oka,M., Dotto,G.P., Fairman,R.M., Velazquez,O.C. and Herlyn,M. (2003) Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol. Cell. Biol. 23 (1), 14-25.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-405

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry