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Primary Antibodies > Stem Cell Antibodies

DELTA-4 Antibody

Rabbit Polyclonal

Code

600-401-696

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's Affinity Purified anti-Delta-4 antibody shows detection of a 74-kDa band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 µg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

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Synonyms: DELTA-4, delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor

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DELTA-4 Antibody Properties

Description

Anti-DELTA-4 (RABBIT) Antibody - 600-401-696

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, partial reactivity to mouse and rat

Clonality

Polyclonal

Application

ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 10 µg/mL to 20 µg/mL
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

0.93 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

DLL4

DELTA-4 Antibody Description

Background

DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Immunogen

Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.

Purity/Specifity

DELTA-4 affinity purified antibody is directed against human DELTA-4. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Lauret,E., et al. (2004) Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential. Leukemia 18 (4), 788-797. Tohda,S., Murata-Ohsawa,M., Sakano,S. and Nara,N. (2003) Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int. J. Oncol. 22 (5), 1073-1079. Liu,Z.J., Shirakawa,T., Li,Y., Soma,A., Oka,M., Dotto,G.P., Fairman,R.M., Velazquez,O.C. and Herlyn,M. (2003) Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol. Cell. Biol. 23 (1), 14-25.

Specific Reference

Indraccolo S, Minuzzo S, Masiero M, et al. Cross-talk between tumor and endothelial cells involving the Notch3-Dll4 interaction marks escape from tumor dormancy. Cancer Res. 2009 Feb 15;69(4):1314–1323. Wu S, Esumi S, Watanabe K, et al. Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon. Development. 2011 Jun;138(12):2499–2509. Zhang Z, Zeng B, Zhang Z, et al. Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression. Cancer Res. 2009 Feb 15;69(4):1578–1586. Lauret,E., et al. (2004) Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential. Leukemia 18 (4), 788-797. Tohda,S., Murata-Ohsawa,M., Sakano,S. and Nara,N. (2003) Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int. J. Oncol. 22 (5), 1073-1079. Liu,Z.J., Shirakawa,T., Li,Y., Soma,A., Oka,M., Dotto,G.P., Fairman,R.M., Velazquez,O.C. and Herlyn,M. (2003) Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol. Cell. Biol. 23 (1), 14-25.

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra

ELISA