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Primary Antibodies  >  Phospho Specific Antibodies
 

Phosphotyrosine Antibody

Mouse Monoclonal 13F9 IgG1 kappa
Code

500-301-262
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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ELISA of Mouse anti-phosphotyrosine antibody.  
Antigen:  BSA conjugates of pT, pY and pS.
Coating amount:  0.1 µg per well.  
Primary antibody:  Phosphotyrosine antibody at 1:1000.  
Dilution series (X-axis):  2-fold. 
Secondary antibody:  HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. 
Substrate:  TMB (p/n TMBE-0100).
Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9).  
Lane 1:  phosphorylated EGFR in an A431 cell lysate.  
Lane 2:  total protein in the unstimulated A431 cells lysate after coommassie staining.  
Load:  10 µg per lane.
Primary antibody:  phosphotyrosine antibody at 1:1000 for 1 hr at RT.
Secondary antibody: IRDye™800 Conjugated Goat-a-Mouse IgG [H&L] (610-132-120) diluted 1:2,500 for 30' at room temperature.
Block:  5% BLOTTO overnight at 4°C.
Other band(s): phosphotyrosine splice variants and isoforms.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: PhosphoTyrosine reactive antibody, monoclonal phosphotyrosine, anti-phosphotyrosine produced in mouse, Mouse anti-phosphotyrosine

Phosphotyrosine Antibody Properties

Description
Anti-PhosphoTyrosine (Ascites) (MOUSE) Monoclonal Antibody - 500-301-262
Host
Mouse
Known Cross Reactivity
phosphotyrosine
Clonality
Monoclonal 13F9 IgG1 kappa
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
ASCITES
Label
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

Phosphotyrosine Antibody Description

Immunogen
This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.
Immunogen Type
Other
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This monoclonal antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation.    Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
Purity/Specifity
This product is clarified ascites produced in balb/c mice using clone 13F9.   Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Sarmay, G., et al., (1994) Protein-tyrosine kinase activity tightly associated with human type II Fc gamma receptors. Proc. Natl. Acad. Sci. USA 91, 4140. Arad-Dann, H., (1993) Immunohistochemistry of phosphotyrosine residues: identification of distinct intracellular patterns in epithelial and steroidogenic tissues. J. Histochem. Cytochem. 41, 513. Dvir, A., et al., (1991) The inhibition of EGF-dependent proliferation of keratinocytes by tyrphostin tyrosine kinase blockers. J. Cell Biol. 113, 857.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human. mouse, rat, monkey
Catalog Number
200-301-269S

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, rat
Catalog Number
200-401-A43

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry