14-12_peptide top banner
 
Secondary Antibodies  >  Phycoerythrin Conjugated Antibodies
 

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Donkey Polyclonal
NCI Collaboration
Order Offline
Number:
710-708-124
Size:
500 µg
Price:
$269.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed Properties

Description
F(ab')2 Anti-MOUSE IgG [H&L] (DONKEY) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins - 710-708-124
Host
Donkey
Specificity
IgG (H&L)
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
IF Microscopy : 1:100 - 1:250
Flow Cytometry : 1:100 - 1:250
Other Dilution: FLOW 1:100 - 1:250
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG F(ab')2
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA:
Lot#  
Print

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed Description

Background
F(ab')2 Anti-Mouse IgG whole molecule generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Synonyms
PE Conjungated Second Antibody, F(ab')2 Mouse Antibody, Dk-a-Mouse IgG, Mouse Antibody in Donkey, F(ab')2 Mouse Secondary Antibody
Immunogen
F(ab')2 Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Purity/Specifity
F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS