House Buffer
 
You may also want to consider these related products.
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Blocking Buffer for Fluorescent Western Blotting - MB-070
Blocking Buffer for Fluorescent Western Blotting - MB-070
Secondary Antibodies  >  Phycoerythrin Conjugated Antibodies
 

F(ab')2 Sheep IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Donkey Polyclonal
Code

713-708-168
Size

500 µg
Price

$269.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Sheep IgG (H&L) Antibody, Dk-a-Sheep Phycoerythrin conjugated, Sheep IgG (H&L) F(ab')2 Antibody in donkey, Donkey F(ab')2 IgG (H&L) Phycoerythrin conjugated Secondary Antibody.

F(ab')2 Sheep IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed Properties

Description
F(ab')2 Anti-SHEEP IgG [H&L] (DONKEY) Antibody Phycoerythrin conjugated Min X Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins - 713-708-168
Host
Donkey
Specificity
IgG (H&L)
Target Species
Sheep
Known Cross Reactivity
Sheep
Clonality
Polyclonal
Application
IF Microscopy : 1:100 - 1:250
FlowCytometry : 1:100 - 1:250
Other Dilution: FLOW 1:100 - 1:250
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG F(ab')2
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

F(ab')2 Sheep IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed Description

Background
Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Immunogen
Anti-Sheep IgG was produced by repeated immunization with sheep IgG whole molecule in donkey.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Application Note
Antibody Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Optimal titers for other applications should be determined by the researcher.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS