Sepharose™ Protein A
SEPHAROSE™ Protein A
SEPHAROSE™ Protein A - Antibody Figure
Immunoprecipitation using Protein A
Immunoprecipitation using Protein A
Immunoprecipitation using Protein A
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Sepharose™ Protein A

11 References
PA50-00-0002 PA50-00-0005 PA50-00-0025
2 mL 5 mL 25 mL
IP
Staphylococcus aureus
$259.00 /Per Item
$409.00 /Per Item
$1,225.00 /Per Item
2 mL $259.00
5 mL $409.00
25 mL $1,225.00
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Background

Sepharose™ Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.

Product Details

Sepharose™ Protein A - PA50-00-0002
ProA, Staphylococcus A protein, Sepharose Protein A
Staphylococcus aureus
Sepharose™
Native Protein

Target Details

Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose™ Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
UniProtKB - P38507

Application Details

IP  - View References
Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Formulation

Suspension of agarose beads
6.0 mg/cc drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
20% (v/v) Ethanol
None
None

Shipping & Handling

Wet Ice
Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE.
Expiration date is one (1) year from date of receipt.
(). Investigating the impact of the phosphorylation status of tyrosine residues within the TACC domain of TACC3 on microtubule behavior during axon growth and guidance. Cytoskeleton (Hoboken).
Applications
IP, Co-IP
(). Abelson-induced phosphorylation of TACC3 modulates its interaction with microtubules and affects its impact on axon outgrowth and guidance. bioRxiv
Applications
IP, Co-IP
(). RNAs interact with BRD4 to promote enhanced chromatin engagement and transcription activation. Nature Structural & Molecular Biology
Applications
IP, Co-IP
(). Mutant p53 shapes the enhancer landscape of cancer cells in response to chronic immune signaling. Nature Communications
Applications
IP, Co-IP
(). Cyclin E/Cdk2-dependent phosphorylation of Mcl-1 determines its stability and cellular sensitivity to BH3 mimetics. Oncotarget
Applications
IP, Co-IP
(). CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire. Nat Commun.
Applications
IP, Co-IP
(). Rho GTPase-independent regulation of mitotic progression by the RhoGEF Net1. Molecular Biology of the Cell
Applications
IP, Co-IP
(). Disrupted junctional membrane complexes and hyperactive ryanodine receptors after acute junctophilin knockdown in mice. Circulation
Applications
IP, Co-IP
(). Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure. Circulation
Applications
IP, Co-IP
(). Respiratory syncytial virus infection alters surfactant protein A expression in human pulmonary epithelial cells by reducing translation efficiency. Am J Physiol Lung Cell Mol Physiol.
Applications
IP, Co-IP
(). Phosphoproteomic analysis of the human pathogen Trypanosoma cruzi at the epimastigote stage. Proteomics
Applications
WB, IB, PCA

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If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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