House Buffer
 
You may also want to consider these related products.
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody - 200-101-147
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody - 200-101-147
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody Peroxidase Conjugated - 200-103-147
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody Peroxidase Conjugated - 200-103-147
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody Biotin Conjugated - 200-106-147
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody Biotin Conjugated - 200-106-147
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Primary Antibodies  >  Serum Protein Component Antibodies
 

Alpha-1-Trypsin Antibody

Rabbit Polyclonal
Code

600-401-147
Size

1 mg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot.  
Lane 1:  molecular weight.  
Lane 2:  Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and  Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST.   
Load:  35 µg per lane.
Primary antibody:  Each primary antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 at 30 min RT.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: A1A antibody, A1AT, AAT, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha-1-AT antibody, alpha1 PI antibody, alpha1 proteinase inhibitor antibody

Alpha-1-Trypsin Antibody Properties

Description
Anti-Human ALPHA-1-ANTI-TRYPSIN (RABBIT) Antibody - 600-401-147
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:600,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.41 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SERPINA1
Download CofA
Lot#  

Alpha-1-Trypsin Antibody Description

Background
ALPHA-1-ANTI-TRYPSIN Antibody is specific for alpha-1-anti-trypsin. Alpha 1-Antitrypsin or a1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily and is generally known as serum trypsin inhibitor. Alpha-1-anti-trypsin protects tissues from enzymes of inflammatory cells. A1AT antibody is suitbale for immunology and cardiovascular researcher.
Immunogen
Human Alpha-1-anti-Trypsin
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody has been assayed against 1.0 µg of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Purity/Specifity
Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins attached to a solid phase.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.  Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-101-147

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
200-103-147

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-106-147

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry