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Proteins and Peptides  >  Cytokines and other Growth Factors
 

TNF-a Human Recombinant Protein

Other - E.coli
NCI Collaboration
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SDS-PAGE shows bands corresponding to TNFa  (1µg) in lane 1 (unreduced, arrowhead) and lane 3 (reduced).   Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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Number:
009-001-306
Size:
50 µg
Price:
$225.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

TNF-a Human Recombinant Protein Properties

Description
TNF-a Human Recombinant Protein - 009-001-306
Host
Other - E.coli
Known Cross Reactivity
Human
Application
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping Condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
TNFA
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TNF-a Human Recombinant Protein Description

Background
TNFa is secreted by macrophages, monocytes, neutrophils, T-cells, and NK- cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha, while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Daltons.
Synonyms
Tumor necrosis factor, TNF-alpha, TNF-a, TNFa, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
TNFa is suitable as a control for polyclonal or monoclonal anti-TNFa in immunological assays.
Purity/Specifity
Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry