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Primary Antibodies  >  Transcription Factor Antibodies
 

PPAR Delta Antibody

Rabbit Polyclonal
Code

600-401-420
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Rabbit Anti-PPAR delta (N terminal specific) 1:500
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: MGC3931 antibody, NR1C2 antibody, NUC1 antibody, NUCI antibody, NUCII antibody, Nuclear hormone receptor 1 antibody, Nuclear receptor subfamily 1 group C member 2 antibody, Peroxisome proliferative activated receptor delta antibod

PPAR Delta Antibody Properties

Description
Anti-PPAR delta (N terminal specific) (RABBIT) Antibody - 600-401-420
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:600,000
Western Blot : 1:500 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.57 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PPARD
Download CofA
Lot#  

PPAR Delta Antibody Description

Background
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.  Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 43 kDa in size corresponding to PPAR delta by western blot in the appropriate tissue or cell lysate.
Purity/Specifity
This affinity purified antibody is directed against mouse PPAR delta protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined.  No reactivity is expected against other subtypes of PPAR.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Mukherjee, R., et al. (1994) Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators. J. Steroid Biochem. Mol. Biol. 51(3-4):157-166. Plutzky, J. (2003) PPARs as therapeutic targets: reverse cardiology? Science 302(5644):406-407. Lee, C.H., et al. (2003) Transcriptional repression of atherogenic inflammation: modulation by PPAR delta. Science 302 (5644):453-457. Chinetti, G., et al. (2000) Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation. Inflamm. Res. 49(10):497-505. Lazar, M.A. (2001) Progress in cardiovascular biology: PPAR for the course. Nat. Med. 1:23-24.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, macaque
Catalog Number
600-401-418

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat, squirrel, orangutan, duck, macaque, boar, mink, guinea pig, rabbit, dog, hamster
Catalog Number
600-401-419

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
mouse, rat, bovine, dog, golden hamster, boar
Catalog Number
600-401-421

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken
Catalog Number
600-401-919

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry