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Primary Antibodies  >  Transcription Factor Antibodies
 

CEBP Delta Antibody

Rabbit Polyclonal
Code

600-401-A61
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Anti-C/EBPd Antibody 
Lane 1: human C/EBPbeta
Lane 2: Mouse C/EBPdelta
Lane 3: untransfected
Lane 4: human C/EBPdelta
Load:  35 µg per lane
Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C 
Blocking: buffer TBST
ECL was used for detection of overexpressed protein in human and mouse C/EBP delta.
Predicted/Observed size:  28.8 kDa, ~35 kDa
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody

CEBP Delta Antibody Properties

Description
Anti-C/EBP delta (RABBIT) Antibody - 600-401-A61
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, human
Clonality
Polyclonal
Application
ELISA : 1:4,000 – 1:9,000
Western Blot : 1:1,000 - 1:15,000
ImmunoPrecipitation: 0.8 – 1.5 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.33 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CEBPD
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Lot#  

CEBP Delta Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.  Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression.  Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T).  Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity.  C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, western blotting, and IHC.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein.  A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence.   No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Osada S., Yamamoto H., Nishihara T., and Imagawa M.  (1996)  DNA binding specificity of the CCAAT/enhancer-binding protein transcription factor family. J Biol Chem. 271(7):3891-6 Thangaraju M., Rudelius M., Bierie B., Raffeld M., Sharan S., Hennighausen L., Huang A.M., and Sterneck E. (2005) C/EBPd is a crucial regulator of pro-apoptotic gene expression during mammary gland involution. Development 132 (21), 4675-85. Huang A.M., Montagna C., Sharan S., Ni Y., Ried T., and Sterneck E. (2004) Loss of CCAAT/enhancer binding protein delta promotes chromosomal instability. Oncogene 23(8):1549-57.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-161

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-162

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-163

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-167

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry