House Phospho Banner
 
You may also want to consider these related products.
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Anti-TGF beta 1 (RABBIT) Antibody - 600-401-432
Anti-TGF beta 1 (RABBIT) Antibody - 600-401-432
Anti-SMAD3 pS423 pS425 (RABBIT) Antibody - 600-401-919
Anti-SMAD3 pS423 pS425 (RABBIT) Antibody - 600-401-919
Anti-SMAD3 (RABBIT) Antibody - 600-401-920
Anti-SMAD3 (RABBIT) Antibody - 600-401-920
Primary Antibodies  >  Transcription Factor Antibodies
 

SMAD3 phospho T179 Antibody

Rabbit Polyclonal
Code

600-401-C48
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
The SMAD pathway follows  the canonical TGF-ß signaling pathway.  TGF-ß dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD (R-SMAD).i.e. SMAD2 or SMAD3.  The R-SMAD then binds to the common SMAD (coSMAD) i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.
NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179.  The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 (RABBIT) Antibody.  The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody

SMAD3 phospho T179 Antibody Properties

Description
Anti-SMAD3 pT179 (RABBIT) Antibody - 600-401-C48
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:15,000-1:75,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.97 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SMAD3
Download CofA
Lot#  

SMAD3 phospho T179 Antibody Description

Background
SMAD3 pT179 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily.  Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway.  The phosphorylated R-Smads are translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Immunogen
Anti-SMAD3 pT179 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-SMAD3 pT179 has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Purity/Specifity
Anti-SMAD3 pT179 affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot.  Expect reactivity against phosphorylated Smad2.  Reactivity against other phosphorylated Smad family members is not known.  A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wang G, Matsuura I, He D, Liu F. 2009 Transforming growth factor-{beta}-inducible phosphorylation of Smad3. J Biol Chem. Apr 10; 284(15):9663-73.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-432

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken
Catalog Number
600-401-919

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken
Catalog Number
600-401-920

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry