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Primary Antibodies > Ubiquitin and UBL Antibodies

Cbl-c Antibody

Rabbit Polyclonal

Code

600-401-889

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Immunoprecipitation and western blot using Rockland's Affinity Purified anti-Cbl-c antibody shows detection of a pre-dominant band at ~52 kDa corresponding to Cbl-c (arrowhead) in transfected cell lysates (left panel). Lysates are from Hek 293T cells transfected with empty vector or with Cbl-c. The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The right panel shows western blotting after first immunoprecipitating with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.

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Synonyms: Cas Br M (murine) ectropic retroviral transforming sequence c antibody, Casitas B lineage lymphoma c antibody, CBL 3 antibody, CBL SL antibody, RING finger protein 57 antibody, RNF57 antibody, SH3 binding protein CBL C antibody

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Cbl-c Antibody Properties

Description

Anti-cbl-c (RABBIT) Antibody - 600-401-889

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 1:500 - 1:3,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.42 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

CBLC

Cbl-c Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57. Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cbl-c knock out mice show no obvious phenotype. Thus, the physiological role of Cbl-c is not known.

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 444-458 of Human Cbl-c.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, immunoprecipitation and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~52 kDa in size corresponding to Cbl-c by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against human Cbl-c protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with Cbl-c from human and chimpanzee sources. Expect partial reactivity against mouse and rat sources of Cbl-c as ~83% sequence homology is on record for the immunogen sequence. Reactivity with Cbl-c from other sources has not been determined. No reactivity is expected with Cbl-a or Cbl-b.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Blake,T.J., Shapiro,M., Morse,H.C. III and Langdon,W.Y. (1991) The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif. Oncogene 6 (4), 653-657. Keane,M.M., Rivero-Lezcano,O.M., Mitchell,J.A., Robbins,K.C. and Lipkowitz,S. (1995) Cloning and characterization of cbl-b: a SH3 binding protein with homology to the c-cbl proto-oncogene. Oncogene 10 (12), 2367-2377. Kim,M., Tezuka,T., Suziki,Y., Sugano,S., Hirai,M. and Yamamoto,T. (1999) Molecular cloning and characterization of a novel cbl-family gene, cbl-c. Gene 239 (1), 145-154.

Specific Reference

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