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Primary Antibodies  >  Ubiquitin and UBL Antibodies
 

Ubiquitin-Conjugating Enzyme E2 J2 Antibody

Rabbit Polyclonal
Code

600-401-B43
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Most modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid).  Arrowheads point to the cleavage sites.  Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.  Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers.  Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination.  In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains.  UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail.  Whether HUB1 functions as a modifier is currently unclear.  APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.  (From Jentsch & Pyrowolakis (2000); see references below.)
Western blot using Rockland's affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over-expressing Myc-Ube2j2 (Lane 1).  Lane 2 contains lysate from mock-transfected 293 cells.  Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD
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Synonyms: Ubiquitin-conjugating enzyme E2 J2 Non-canonical ubiquitin-conjugating enzyme 2 Ube2j2

Ubiquitin-Conjugating Enzyme E2 J2 Antibody Properties

Description
Anti-Ubiquitin-Conjugating Enzyme E2 J2 (Ube2j2) [RABBIT) Antibody - 600-401-B43
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.91 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
UBE2J2
Download CofA
Lot#  

Ubiquitin-Conjugating Enzyme E2 J2 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j2 and Ube2j1 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal trans-membrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against human Ube2j2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lenk,U., Yu,H., Walter,J., Gelman,M.S., Hartmann,E., Kopito,R.R. and Sommer,T. (2002) A role for mammalian Ubc6 homologues in ER-associated protein degradation. J. Cell Sci. 115:3007-3014. Lester,D., Farquharson,C., Russell,G. and Houston,B. (2000) Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. Biochem. Biophys. Res. Commun. 269(2):474-480. Jentsch, S. and Pyrowolakis, G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-342.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry