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Secondary Antibodies  >  Fluorescein Conjugated Antibodies
 

Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

Goat Polyclonal
Code

610-102-121
Size

1 mg
Price

$179.00
Availability

Ships next business day.
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E-mail: orders@rockland-inc.com
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Dot Blot showing the detection of Mouse IgG.  A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose.  After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (p/n 610-102-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Western Blot of Goat anti-Mouse IgG Pre-Adsorbed Fluorescein Conjugated Secondary Antibody.  
Lane 1:  Mouse IgG.  
Lane 2:  None.  
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  25 & 55 kDa, 25 & 55 kDa for Mouse IgG. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: FITC Conjugated Secondary Antibody, Anti Mouse IgG FITC, Gt Anti-Mouse, GAM

Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed Properties

Description
Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-102-121
Host
Goat
Specificity
IgG (H&L)
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
FlowCytometry : 1:200 - 1:500
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
3.7 moles Fluorescein (FITC) per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed Description

Background
FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
Mouse IgG whole molecule
Storage Condition
Store anti mouse secondary antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Secondary antibody reagents are ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods.
Purity/Specifity
Secondary antibody to Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate