House Buffer
 
You may also want to consider these related products.
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - MB-064-1000
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - MB-064-1000
Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

MOUSE IgG (H&L) Secondary Antibody Peroxidase Conjugated

Rabbit Polyclonal
Code

610-4302
Size

2 mg
Price

$129.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Rockland's Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size)., and blocked with 5% BLOTTO (p/n B501-0500) o/n at 4° C. The membrane was then probed with a 1:2,500 dilution of HRP Rb-a-Mouse IgG [H&L] (p/n 610-4302) for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110). Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning:  5X5.  Exposure time:  2 min.  Aperture open.  No emission or excitation filters used.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: hrp mouse antibody, anti mouse igg hrp, conjugated secondary antibody

MOUSE IgG (H&L) Secondary Antibody Peroxidase Conjugated Properties

Description
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Host
Rabbit
Specificity
IgG (H&L)
Target Species
Mouse
Clonality
Polyclonal
Application
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:1,000 - 1:5,000
Immunochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

MOUSE IgG (H&L) Secondary Antibody Peroxidase Conjugated Description

Background
Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Immunogen
Mouse IgG whole molecule
Storage Condition
Store mouse second antibody conjugate at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment
Purity/Specifity
HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Sone, H., & Kagawa, Y. (2005). Pancreatic beta cell senescence contributes to the pathogenesis of type 2 diabetes in high-fat diet-induced diabetic mice. Diabetologia, 48(1), 58-67.
Log in to submit your product review.

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate