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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

RABBIT IgG (H&L) Antibody Pre-adsorbed

Goat Polyclonal
Code

611-101-122
Size

1 mg
Price

$105.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed.
Lane 1:  Rabbit IgG.
Lane 2:  None.
Load:  100 ng per lane.
Primary antibody:  RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4°C.
Secondary antibody: DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  55 and 28 kDa, 55 and 28 kDa for Rabbit IgG.
Other band(s): none.
SDS-Page of Goat anti-Rabbit IgG (H&L) Pre-adsorbed Secondary Antibody.
Lane 1:  Goat anti-Rabbit IgG – Non-Reduced.
Lane 2:  Goat anti-Rabbit IgG – Reduced.
Load:  1.0 µg per lane.
Predicted/Observed size:  25 & 55 kDa – Reduced, 160 kDa – Non-Reduced for Goat IgG.
Other Band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Rabbit Antibody, Gt-a-Rabbit IgG, Rabbit Antibody in Goat, Rabbit Secondary Antibody

RABBIT IgG (H&L) Antibody Pre-adsorbed Properties

Description
Anti-RABBIT IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-101-122
Host
Goat
Specificity
IgG (H&L)
Target Species
Rabbit
Known Cross Reactivity
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:25,000 - 1:100,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

RABBIT IgG (H&L) Antibody Pre-adsorbed Description

Background
Anti-Rabbit IgG antibody generated in goat detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Application Note
Anti-Rabbit IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  Specificity was confirmed using ELISA against at less than 1% of target signal.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry