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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated

Goat Polyclonal
Code

611-1302
Size

2 mg
Price

$115.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody.
Lane 1:  HeLa Whole Cell Lysate. 
Lane 2:  NIH/3T3 Whole Cell Lysate.  
Load:  10 µg per lane.
Primary antibody:  Beta Actin Antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  42 kDa, 42 kDa.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: rabbit HRP antibody, horseradish peroxidase conjugated secondary antibodies

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Properties

Description
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Host
Goat
Specificity
IgG (H&L)
Target Species
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:200,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Description

Background
Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) is available in a variety of formats. HRP Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other HRP antibody based assays.
Immunogen
Rabbit IgG whole molecule
Storage Condition
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Purity/Specifity
RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate