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Recombinant proteins are frequently produced with a poly-Histidine epitope tag to allow for detection and affinity purification. These “HIS-tagged” recombinant proteins may be produced in many different host species including E. coli, insect, yeast, or mammalian cells. The 6X His tag is typically located at either the amino or carboxyl terminus (also called N-terminal HIS tag or C-terminal HIS tag). Rockland’s Anti-6X Histidine affinity tag antibodies are highly specific to polyhistidine epitope tags used when producing recombinant protein. Rockland’s anti-6X HIS antibody can bind to the typical 6X HIS, but can also bind to less frequently used larger 8X or 10X histidine epitopes. These antibodies are excellent for detection of 6X HIS tagged recombinant proteins. Featured Products:
Anti-6X HIS EPITOPE TAG
Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody
Anti-6X HIS EPITOPE TAG (RABBIT) Antibody IRDye® 700DX Conjugated
Rockland manufactures and validates anti-6X histidine antibodies against multiple recombinant proteins tagged at the amino or carboxyl terminus. The antibodies are characterized for robust specific activity. Rockland also produces anti-6X HIS antibodies as peroxidase enzyme or dye reporter conjugates, providing the greatest flexibility to the end user in the design of their assay. Rigorous quality control testing ensures that the final product meets or exceeds Rockland’s standards for robust performance in your assays.
Anti-6X HIS EPITOPE TAG Antibody
Anti-6X HIS EPITOPE TAG Antibody Peroxidase Conjugated
Anti-6X HIS EPITOPE TAG Antibody IRDye® 700DX Conjugated
Anti-6X HIS EPITOPE TAG Monoclonal Antibody
Anti-6X HIS EPITOPE TAG Monoclonal Antibody IRDye® 800 Conjugated
WB, IP, ELISA
6X HIS antibodies can be used but are not limited to the following assays:
• Western blot• Immunoprecipitation• Dot blot• ELISASee Rockland’s other epitope tag antibodies such as anti-FLAG and anti-GST.Rockland also offers cell lysates and extracts suitable for use as controls for HIS tag detection in control experiments and Rockland offers custom molecular biology services for the production or recombinant proteins contain HIS tag.
Rockland'sTrueBlot® product line enables researchers to generate publication-quality IP western blot data. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot®, Mouse TrueBlot®, Rabbit TrueBlot® or Sheep TrueBlot®.
TrueBlot® is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625