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Guinea Pig IgG Fab (BULK ORDER)

NCI Collaboration
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Number:
006-0105
Size:
5 mg
Price:
Price On Request
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

Guinea Pig IgG Fab (BULK ORDER) Properties

Description
GUINEA PIG IgG Fab fragment (BULK ORDER) - 006-0105
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Guinea Pig
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG Fab
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
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Guinea Pig IgG Fab (BULK ORDER) Description

Background
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.
Synonyms
Human Immunoglobulin Fab, F(ab), Fragment antigen-binding
Storage Condition
Store vial at 4° C prior to restoration.   Restore with 5.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Guinea Pig IgG Fab fragment is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Guinea Pig IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Purity/Specifity
Guinea Pig IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Guinea Pig IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum, anti-Guinea Pig IgG and anti-Guinea Pig IgG F(ab’)2.  No reaction was observed against anti-Guinea Pig IgG F(c) or anti- Papain.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
010-0005

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
011-0105

Product Type
Proteins and Peptides;
Catalog Number
012-0305

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
106-4104

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry