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Armenian Hamster IgG isotype Control

Hamster Armenian
NCI Collaboration JCYIA Product
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Number:
019-001-002
Size:
1 mg
Price:
$113.00
Shipping:
$32.00
Handling:
$20.00
Availability:
Ships next business day.

Armenian Hamster IgG isotype Control Properties

Description
ARMENIAN HAMSTER IgG whole molecule isotype control - 019-001-002
Host
Hamster Armenian
Application
FLISA : User Optimized
IF Microscopy : User Optimized
Flow Cytometry : 1:1000-1:5000
Other Dilution: User Optimized
Species of Origin
Armenian Hamster
Physical State
Lyophilized
Shipping Condition
Wet Ice
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Armenian Hamster IgG isotype Control Description

Background
Armenian Hamster isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In hamster there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and µ - IgM, light chain kappa and lambda.
Synonyms
Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
ARMENIAN HAMSTER IgG whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.
Purity/Specifity
ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG.  Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Stadlmann J, Pabst M, Kolarich D, Kunert R, Altmann F. (2008). "Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides". Proteomics 8 (14): 2858–2871.
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Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry