Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Cdk4 Antibody

Rabbit Polyclonal
NCI Collaboration JCYIA Product
Order Offline
Number:
100-401-162
Size:
100 µL
Price:
$339.00
Shipping:
$41.00
Handling:
$20.00
Availability:
Ships next business day.

Cdk4 Antibody Properties

Description
Anti-cdk4 (RABBIT) Antibody - 100-401-162
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, rat and mouse
Clonality
Polyclonal
Application
ELISA : 1:500 - 1:2,000
Western Blot : 1:200 - 1:500
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDK4
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Cdk4 Antibody Description

Background
Cyclin-dependent kinase 4 is a Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. It hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. It also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Cdk4 is a component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Synonyms
rabbit anti-Cdk4 Antibody, cdk, CDK4 protein antibody, Cell division kinase 4 antibody, Cell division protein kinase 4 antibody, CMM 3 antibody, Melanoma cutaneous malignant 3 antibody, MGC14458 antibody, p34 cdk4 antibody, cyclin dependent kinase 4
Immunogen
cdk4 (p34) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Anti-Cdk4 is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 34 kDa cdk4 (p34) protein from human, rat and mouse tissue.  No reaction was observed against other related cyclin dependent kinases.  Cross reactivity with cdk4 (p34) from other species has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-153

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, rat and mouse
Catalog Number
100-401-161

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, rat and mouse
Catalog Number
100-401-163

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
A Broad Range Of Species
Catalog Number
200-401-431

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry