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Datasheet

SIX3 Antibody

Guinea Pig Polyclonal

5 References
200-201-A26S 200-201-A26
25 µL 50 µg
WB, IHC, IF, Dot Blot, Multiplex
Mouse
Guinea Pig
$125.00 /Per Item
$475.00 /Per Item
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Background

Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke’s pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Product Details

Anti-Homeobox protein SIX3 (GUINEA PIG) Antibody - 200-201-A26
guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3
Guinea Pig
Polyclonal
IgG

Target Details

Six3  - View All Six3 Products
Mouse
Conjugated Peptide
This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
This product was purified by Protein A chromatography from monospecific antiserum.  This antibody reacts with mouse Six3.   Cross-reactivity with Six3 from other sources has not been determined.

Application Details

Dot Blot, IF, IHC, WB
Multiplex  - View References
This Protein A purified antibody has been tested for use in Immunohistochemistry, immunofluorescence microscopy, dot blot, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Formulation

Liquid (sterile filtered)
0.65 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus. Development.
Applications
IHC, ICC, Histology; Multiplex Assay
(). Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs. Cell Death & Disease
Applications
IHC, ICC, Histology
(). Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies. PLOS Genetics
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Mesencephalic origin of the rostral Substantia nigra pars reticulata. Brain Structure and Function
Applications
IHC, ICC, Histology
(). Molecular regulation of the developing commissural plate. The Journal of Comparative Neurology
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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